NOVEL C TYPE LECTIN FOLD IMPLICATIONS ON NK CELL ASSOCIATED CD94 & NKG2 RECEPTOR

新型 C 型凝集素折叠对 NK 细胞相关 CD94 的影响

• 批准号: 6205785
• 负责人: PETER SUN
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205785
• 关键词: biological products; biomedical resource; enzymes; genetics; proteins; spectrometry; technology /technique

The 16 proteins identified for the pilot project were cloned and expressed in E. Coli. The cell pastes were analyzed for metal content by x-ray spectroscopy on beamline X-9B. P0001 was found to contain zinc, all other proteins were negative for metal content. P0001 is in its native state a Mn containing protein, in the expression system used it coordinated zinc.

克隆了为试点项目鉴定的16种蛋白质， 在大肠杆菌中表达杆菌 分析电池糊的金属含量 通过X-9 B光束线上的X射线光谱学 发现P0001含有 锌，所有其他蛋白质的金属含量均为阴性。 P0001在 在表达系统中，其天然状态为含Mn蛋白质， 用它来配锌