4 MONTH BIOMED GRANT LETTER WAS SENT TO D DEERFIELD

血管紧张素II（AII）受体，其中有两个主要的 亚型AT1和AT2属于G蛋白连接的超家族， 受体（Gr）。 了解AII的系统发育关系 受体与该家族的其他成员可以提供洞察力， 这些受体的作用和调节机制。 在阐明这些行动方面取得了重大进展， AT1受体的机制，但AT2亚型的机制仍然存在 一个谜 我们之前的努力是为了确定 基于部分氨基酸的AII受体与其他Gr的关系 序列，一直模棱两可。 为了获得更多的视角， AII受体亚型与其他Gr， 这项提案计划检查核酸序列。 这 信息可能会导致一个更明确的系统发育 AII受体亚型的表征。 第二个方面 项目是确定AII上潜在的磷酸化位点 受体的这个项目是确定潜在的磷酸化 AII受体上可能参与受体 脱敏。 AII胞内结构域中的氨基酸 受体将与其他Gr的受体进行比较， 通过磷酸化去敏化。

Angiotensin II (AII) receptors, of which there are two major subtypes, AT1, and AT2, belong to a superfamily of G protein-linked receptors (Gr). Knowledge of the phylogenetic relationships of AII receptors with other member of this family can provide insight into the mechanisms of action and regulation of these receptors. Substantial progress has been made to elucidate the actions and mechanisms of the AT1 receptors, but those of the AT2 subtypes remain an enigma. Our previous efforts to determine the phylogenetic relationship of AII receptors to other Gr based on partial amino acid sequences, have been ambiguous. To gain an additional perspective on the phylogenetic relationships of AII receptor subtypes and other Gr, this proposal plans to examine nucleic acid sequences. This information may lead to a more definitive phylogenetic characterization of AII receptor subtypes. A second aspect of this project is to determine potential phosphorylation sites on the AII receptors of this project is to determine potential phosphorylation sites on the AII receptors that may be involved in receptor desensitization. Amino acids in the intracellular domains of AII receptors will be compared with thos of other Gr that undergo densensitization by phosphorylation.