4 MONTH BIOMED GRANT LETTER WAS SENT TO D DEERFIELD

4 个月的 Biomed 授权信已发送给 D DEERFIELD

• 批准号: 6221077
• 负责人: SAMUEL NEFF
• 金额: $ 0.13万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6221077
• 关键词: animal tissue; biomedical resource; cardiovascular system; drug adverse effect; endocrine gland /system; nervous system; proteins; reproductive system

Angiotensin II (AII) receptors, of which there are two major subtypes, AT1, and AT2, belong to a superfamily of G protein-linked receptors (Gr). Knowledge of the phylogenetic relationships of AII receptors with other member of this family can provide insight into the mechanisms of action and regulation of these receptors. Substantial progress has been made to elucidate the actions and mechanisms of the AT1 receptors, but those of the AT2 subtypes remain an enigma. Our previous efforts to determine the phylogenetic relationship of AII receptors to other Gr based on partial amino acid sequences, have been ambiguous. To gain an additional perspective on the phylogenetic relationships of AII receptor subtypes and other Gr, this proposal plans to examine nucleic acid sequences. This information may lead to a more definitive phylogenetic characterization of AII receptor subtypes. A second aspect of this project is to determine potential phosphorylation sites on the AII receptors of this project is to determine potential phosphorylation sites on the AII receptors that may be involved in receptor desensitization. Amino acids in the intracellular domains of AII receptors will be compared with thos of other Gr that undergo densensitization by phosphorylation.

血管紧张素 II (AII) 受体，其中有两种主要受体 亚型 AT1 和 AT2 属于 G 蛋白连接超家族 受体（Gr）。 AII 系统发育关系的知识 与该家族其他成员的受体可以提供洞察力 这些受体的作用和调节机制。 在阐明行动和行动方面已取得实质性进展 AT1 受体的机制，但 AT2 亚型的机制仍然存在 一个谜。 我们之前为确定系统发育所做的努力 基于部分氨基酸的AII受体与其他Gr的关系 序列，已经含糊不清。 为了获得额外的视角 AII受体亚型和其他Gr的系统发育关系， 该提案计划检查核酸序列。 这 信息可能会导致更明确的系统发育 AII 受体亚型的表征。 这的第二个方面 项目是确定 AII 上潜在的磷酸化位点 该项目的受体是确定潜在的磷酸化 AII 受体上可能参与受体的位点 脱敏。 AII 胞内结构域中的氨基酸 受体将与其他经历过的 Gr 的受体进行比较 通过磷酸化致密化。