3D MR MICROSCOPY OF NEONATE MUTANT ANKYRINB MICE BRAINS

新生锚定蛋白突变小鼠大脑的 3D 磁共振显微镜

• 批准号: 6122315
• 负责人: VANN BENNETT
• 金额: $ 0.05万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122315
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; genetics; human tissue; magnetic resonance imaging; nervous system

The ankyrinB gene encodes 150, 220, and 440 kDa ankyrinB, which are membrane-associated structural proteins expressed in brain and heart. Biochemical functions of ankyrinB include linkage of members of the L1 family of cell adhesion molecules to spectrin-actin complexes. Physiological functions of ankyrins in brain are not known. We have disrupted the gene encoding ankyrinB in mice, with the consequence that these animals die within 20 days of birth. Brains of mutant animals reveal loss of major nerve tracts and enlarged ventricles, based on histology. We wish to evaluate the three-dimensional structure of the brains of normal and mutant neonatal mice by MR microscopy to better appreciate macroscopic consequences of ankyrinB deficiency, and in particular the details of ventricular enlargement. Animals well be sacrificed and the tissues placed in 4% buffered formaldehyde.

AnkyrinB基因编码150、220和440 kDa的ankyrinB，它 膜相关结构蛋白在大脑中表达， 心。AnkyrinB的生化功能包括成员间的连接 细胞黏附分子L1家族与血影蛋白-肌动蛋白 复合体。Anyrins在脑中的生理功能不是 为人所知。我们已经扰乱了小鼠的ankyrinB编码基因， 后果是这些动物在出生后20天内死亡。我的大脑 突变动物显示主要神经束丢失和扩大 脑室，根据组织学。我们希望评估 正常和突变人大脑的三维结构 对新生小鼠进行磁共振显微镜检查，以更好地了解宏观情况 血凝素B缺乏的后果，尤其是 脑室扩大。动物将被牺牲，组织 放置在4%的缓冲甲醛中。