ROLE OF ANKYRIN B IN CARDIAC DEVELOPMENT & FUNCTION

锚蛋白 B 在心脏发育中的作用

• 批准号: 6282351
• 负责人: VANN BENNETT
• 金额: $ 1.33万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282351
• 关键词: Mammalia; biomedical resource; cardiovascular system; genetics; human tissue; magnetic resonance imaging

Ankyrin-B is a candidate protein to spatially organize IP3 and ryanodine receptors in striated muscles. We have knocked out the gene encoding ankyrin-B in the mouse by homologous recombination, and are now evaluating its consequences for cardiac development. We have found in isolated cardiomyocytes that the IP3 receptor is mis-sorted, and that patterns of calcium release are grossly abnormal. Ankyrin-B depleted hearts of exhibit reduction in ventricular mass, although their ultra structure is essentially normal. The goals of the proposal are to quantitatively assess the effects of ankyrin-B knock out on the volumetric and microstructural composition of the neonate mouse heart by using both conventional 3D and diffusion tensor MR microscopy.

锚蛋白-B是空间组织IP 3的候选蛋白， 横纹肌中的兰尼碱受体 我们已经敲除了 通过同源重组在小鼠中编码锚定蛋白-B， 现在正在评估它对心脏发育的影响。 我们有 在分离的心肌细胞中发现IP 3受体被错误分类， 而且钙的释放模式非常不正常 ankyrin-B 衰竭的心脏表现出心室质量的减少，尽管 超微结构基本正常。 的目标 建议定量评估ankyrin-B敲击的影响 新生儿的体积和微观结构组成 常规3D和扩散张量MR对小鼠心脏的研究 显微镜