3D MR MICROSCOPY OF NEONATE MUTANT ANKYRINB MICE BRAINS

新生锚定蛋白突变小鼠大脑的 3D 磁共振显微镜

• 批准号: 6282350
• 负责人: VANN BENNETT
• 金额: $ 1.33万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282350
• 关键词: Mammalia; biomedical resource; genetics; human tissue; magnetic resonance imaging; nervous system

The ankyrinB gene encodes 150, 220, and 440 kDa ankyrinB, which are membrane-associated structural proteins expressed in brain and heart. Biochemical functions of ankyrinB include linkage of members of the L1 family of cell adhesion molecules to spectrin-actin complexes. Physiological functions of ankyrins in brain are not known. We have disrupted the gene encoding ankyrinB in mice, with the consequence that these animals die within 20 days of birth. Brains of mutant animals reveal loss of major nerve tracts and enlarged ventricles, based on histology. We wish to evaluate the three-dimensional structure of the brains of normal and mutant neonatal mice by MR microscopy to better appreciate macroscopic consequences of ankyrinB deficiency, and in particular the details of ventricular enlargement. Animals well be sacrificed and the tissues placed in 4% buffered formaldehyde.

锚蛋白B基因编码150、220和440 kDa的锚蛋白B， 是在脑中表达的膜相关结构蛋白， 心 锚蛋白B的生化功能包括连接成员 细胞粘附分子L1家族与血影蛋白-肌动蛋白的结合 配合物 锚蛋白在大脑中的生理功能不是 知道的 我们已经破坏了小鼠中编码ankrelin B的基因， 这些动物在出生后20天内死亡。 大脑 突变动物显示主要神经束的丢失和扩大 根据组织学判断是心室 我们希望评估 正常人和突变人大脑的三维结构 新生小鼠通过MR显微镜检查，以更好地了解宏观 B缺乏的后果，特别是细节 心室扩大 将动物处死， 置于4%缓冲甲醛中。