CHAR OF OXYGEN SENSING CAPABILITY & STABILITY OF EPR ACTIVE CHARCOALS

Gloxy是一种煤，其EPR信号非常 对氧分压（pO2）的变化敏感。 它有 成为主要顺磁材料之一的潜力， 将用于扩大EPR血氧测定在生活中的领域 科目 评估组织对存在的 这种材料的有效使用是必不可少的。 的目的 研究目的是确定生物相互作用的组织学证据 的组织的存在植入gloxy和长期 组织对氧的敏感性稳定。 Gloxy是 植入大脑，肾脏，肝脏和四头肌， 小鼠 植入后，将含有gloxy的组织 在特定终点收获，组织学检查 通过光学和电子显微镜进行。 定期EPR 从活体动物获得光谱。 组织学评估 证明了gloxy是相当稳定的，并且没有诱导显著的 本研究期间检查的组织中的反应 观察力 在24小时，一些样品确实有证据表明， 出血似乎与身体创伤有关， 植入和一些炎性细胞也存在。 七 植入后30天，没有出血迹象， 最多观察到少量炎性细胞。 在整个观察期间，没有证据表明 炎症或异物组织反应。 这两种形式的gloxy保持其敏感性，至少对氧 在整个实验（6个月）中，在所有各种器官（ 脑、肾、肝、肌肉）。

Gloxy is a type of coal which has an EPR signal which is very sensitive to changes of the partial pressure of oxygen (pO2). It has the potential to be one of the principal paramagnetic materials that will be used for the widening field of EPR oximetry in living subjects. An assessment of the responses of tissues to the presence of this material is essential for its effective use. The aim of this study is to ascertain histological evidence of biological interaction of the tissues to the presence of the implanted gloxy and long-term stability in tissues of the sensitivity to oxygen. Gloxy was implanted into the brain, kidney, liver, and quadriceps muscle of mice. After implantation, the tissues containing the gloxy were harvested at specific endpoints and histological examination was carried out by light and electron microscopy. Periodically EPR spectra were obtained from the live animals. Histological assessments demonstrated that gloxy is quite stable and did not induce significant reactions in the tissues examined in this study during the time period of observation. At 24 hr. some of the samples did have evidence of hemorrhage which appeared to be associated with the physical trauma of implantation and some inflammatory cells also were present. Seven days after the implantation, there was no indication of hemorrhage and at most, a small amount of inflammatory cells was observed. Throughout the observation period there was no evidence of significant inflammation or foreign tissue reaction at the site of the gloxy. Both forms of gloxy remained their sensitivity to oxygen at least throughout the experiment ( 6 months) in all of the various organs ( brain, kidney, liver, muscle).