BIOLOGICAL INTERACTIONS OF OXYGEN SENSITIVE CHARCOALS

氧敏感木炭的生物相互作用

• 批准号: 6353196
• 负责人: ROBERT B. CLARKSON
• 金额: $ 1.22万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353196
• 关键词: Mammalia; bioengineering /biomedical engineering; biological products; biomedical resource; gas; model design /development; technology /technique

It is expected that the oxygenation status of tissues may be a significant factor in the progression of the healing of wounds. Wound healing is profoundly influenced by local blood supply, vasoconstriction, and all other factors that govern perfusion and blood oxygenation. It is generally believed that impaired perfusion and oxygenation are the most frequent causes of healing failure, and wounds in highly vascularized tissues (e.g., head, anus) heal fast and are quite resistant to infection. The availability of such data, combined with a means to measure the pO2 in healing wounds, would provide a powerful tool to improve clinical treatment of wounds. The group at Northwestern has developed several well characterized models for making wounds and following their healing. The purpose of this collaborative study is to explore the possibility of using EPR oximetry to investigate the role of actual pO2 on critical parameters relevant to wound healing - specifically, to measure the tissue pO2 at different stages of the wound healing process, and at different locations in and around the wound. Studies have been initiated in which paramagnetic particulate materials are inserted into the regions of the wounds and equivalent areas in which no wound is made. The effect of ischemia and aging on pO2 of wounded tissue also is being studied. Using gloxy as the oxygen-sensitive material, we have carried out initial studies with two wound healing models: mouse ischemic flap model, and ischemia rat ear model. By implanting gloxy particles (about 0.5-0.8 mm in diameter) in three different sites in the wounded area of a rat ear, as well as in the opposite control ear, we were able to measure tissue pO2 at different sites by placing the resonator over the region of interest.

预计组织的氧合状态可能是 伤口愈合过程中的重要因素。 伤口 愈合受到局部血液供应的深刻影响， 血管收缩，以及控制灌注和的所有其他因素 血液氧合。 一般认为，灌注受损 和氧合作用是愈合失败的最常见原因，并且 高度血管化组织（例如头部、肛门）的伤口愈合快且 对感染有很强的抵抗力。 此类数据的可用性， 结合测量愈合伤口中 pO2 的方法，将 为改善伤口的临床治疗提供了有力的工具。 这 西北大学的研究小组开发了几种特征良好的模型 用于制造伤口并随后愈合。 这样做的目的 合作研究是探索使用EPR的可能性 血氧测定法研究实际 pO2 对关键参数的作用 与伤口愈合相关 - 具体而言，测量组织 pO2 伤口愈合过程的不同阶段、不同时期 伤口内和周围的位置。 研究已启动于 将哪些顺磁性颗粒材料插入到这些区域中 伤口和未造成伤口的同等区域。 这 缺血和衰老对受伤组织 pO2 的影响也正在研究中 研究过。 使用 Glooxy 作为氧敏感材料，我们有 用两种伤口愈合模型进行了初步研究：小鼠 缺血皮瓣模型、缺血大鼠耳模型。 通过植入 Glooxy 颗粒（直径约0.5-0.8毫米）位于三个不同位置 大鼠耳朵的受伤区域以及另一只对照耳朵的受伤区域， 我们能够通过放置不同位置的组织 pO2 来测量 感兴趣区域上的谐振器。