BIOLOGICAL INTERACTIONS OF OXYGEN SENSITIVE CHARCOALS

预计组织的氧合状态可能是 这是伤口愈合进程中的重要因素。伤口 愈合受到当地血液供应的深刻影响， 血管收缩，和所有其他因素，控制血流和 血液氧合作用。人们普遍认为，血流灌注受损 和氧合是愈合失败的最常见原因，以及 高血管组织(如头部、肛门)的伤口愈合快， 对感染有很强的抵抗力。这些数据的可用性， 结合一种测量愈合伤口中氧分压的方法， 为改善创面的临床治疗提供了有力的工具。这个 西北大学的研究小组开发了几种很有特色的模型 为了制造伤口并追踪伤口的愈合。这样做的目的是 协作学习是为了探索使用EPR的可能性 血氧仪研究实际PO2对关键参数的作用 与伤口愈合相关-具体地说，测量组织在 伤口愈合过程的不同阶段，以及在不同的 伤口内部和周围的位置。研究已在#年启动 哪些顺磁性颗粒材料被插入到这些区域 伤口和未造成伤口的同等区域。这个 缺血和老化对损伤组织氧分压的影响也在研究中 学习。使用Gloxy作为氧气敏感材料，我们有 使用两种伤口愈合模型进行了初步研究：小鼠 缺血皮瓣模型和大鼠缺血耳廓模型。通过植入Gloxy 三个不同地点的颗粒物(直径约0.5-0.8毫米) 大鼠耳朵的损伤区域，以及相对的对照耳朵， 我们能够测量不同部位的组织氧分压 感兴趣区域上的共振器。

It is expected that the oxygenation status of tissues may be a significant factor in the progression of the healing of wounds. Wound healing is profoundly influenced by local blood supply, vasoconstriction, and all other factors that govern perfusion and blood oxygenation. It is generally believed that impaired perfusion and oxygenation are the most frequent causes of healing failure, and wounds in highly vascularized tissues (e.g., head, anus) heal fast and are quite resistant to infection. The availability of such data, combined with a means to measure the pO2 in healing wounds, would provide a powerful tool to improve clinical treatment of wounds. The group at Northwestern has developed several well characterized models for making wounds and following their healing. The purpose of this collaborative study is to explore the possibility of using EPR oximetry to investigate the role of actual pO2 on critical parameters relevant to wound healing - specifically, to measure the tissue pO2 at different stages of the wound healing process, and at different locations in and around the wound. Studies have been initiated in which paramagnetic particulate materials are inserted into the regions of the wounds and equivalent areas in which no wound is made. The effect of ischemia and aging on pO2 of wounded tissue also is being studied. Using gloxy as the oxygen-sensitive material, we have carried out initial studies with two wound healing models: mouse ischemic flap model, and ischemia rat ear model. By implanting gloxy particles (about 0.5-0.8 mm in diameter) in three different sites in the wounded area of a rat ear, as well as in the opposite control ear, we were able to measure tissue pO2 at different sites by placing the resonator over the region of interest.