RAT GLIOMA LACTATE TURNOVER: MEASURED BY IN VIVO NMR SPECTROSCOP: BRAIN NEOPLASM

在过去的两年里，我们专注于探索和建立 宽频带质子去偶的可行性和13 C的评价 在4特斯拉下的光谱。 一种新颖的线圈设计，使我们能够 在低于1.5特斯拉时报告的功率水平下去耦， 介绍，我们证明了在人体肌肉，检测 来自天然丰富谷氨酸盐的窄共振（2-4 Hz线宽）， 肌酸胆碱和牛磺酸 我们建立了完整的三维模型 使用极化转移定位13 C MRS， 被称为质子激发的碳-13图像选择在体内定位 准确地说是光谱学。 我们展示了成功的 在人脑中的三维定位在4 T通过 消除所有头皮脂质信号，并记录显著 在4 T时灵敏度增加（约5倍），超过先前的灵敏度 报告为2.1特斯拉。 代谢物峰的线宽为 在人脑中低至2-3 Hz，这基本上与 那些报告在较低的领域。 13 C MRS也与 用1H MRS将一个异常高的高峰指定给在 通过检测74.5ppm处的升高的强度来检测正常人脑。

In the past two years, we focused on exploring and establishing the feasibility of broadband proton decoupling and evaluating 13C spectroscopy at 4 Tesla. A novel coil design, that has allowed us to decouple at power levels below those reported at 1.5 Tesla was introduced and we demonstrated in human muscle, the detection of narrow resonances (2-4 Hz linewidth) from natural abundance glutamate, creatine, choline, and taurine. We established full three-dimensional localization of 13C MRS using polarization transfer based on which we dubbed Proton Excited Carbon-13 Image Selected in vivo Localized spectroscopy, PRECISELY. We demonstrated the successful three-dimensional localization in the human brain at 4T via the elimination of all scalp lipid signals and documented significant sensitivity increases at 4T (~ factor 5) over those previously reported at 2.1 Tesla. Metabolite peaks had linewidths that were as low as 2-3 Hz in the human brain, which is essentially the same as those reported at lower fields. 13C MRS was also used in conjunction with 1H MRS to assign an unusually high peak to scyllo-inositol in the normal human brain by detecting an elevated intensity at 74.5 ppm.