CDC28 TWO HYBRID SCREENS

CDC28 两种混合屏幕

• 批准号: 6123479
• 负责人: STANLEY FIELDS
• 金额: $ 4.02万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6123479
• 关键词: bioengineering /biomedical engineering; biomedical resource; genome; microorganism; plants; proteins; technology /technique

A screen of the yeast activation domain array was carried out using wild-type Cdc28 and a kinase-dead mutant, Cdc28K30R, as baits. The DNA-binding domain fusion constructs were constructed in the Futcher laboratory and sent to the Resource Center. The first screen of the wild-type Cdc28 bait on -histidine media + 2mM 3-aminotriazole showed a somewhat high His+ background, but 13 positives were clearly visible above background, including three proteins known to interact with Cdc28.

进行酵母激活结构域阵列的筛选， 使用野生型Cdc 28和激酶死亡突变体Cdc 28 K30 R作为诱饵。 DNA-结合结构域融合构建体在大肠杆菌中构建。 Futcher实验室并发送到资源中心。 第一屏幕 在组氨酸培养基+2 mM 3-氨基三唑上的野生型Cdc 28诱饵 显示出有点高的His+背景，但13个阳性明显 在背景上可见，包括三种已知相互作用的蛋白 关于Cdc 28