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CDC28 TWO HYBRID SCREENS

CDC28 TWO HYBRID SCREENS
CDC28 两种混合屏幕
批准号:
6123479
负责人:
STANLEY FIELDS
金额:
$4.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-30 至 1999-08-31

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中文摘要
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英文摘要
A screen of the yeast activation domain array was carried out using wild-type Cdc28 and a kinase-dead mutant, Cdc28K30R, as baits. The DNA-binding domain fusion constructs were constructed in the Futcher laboratory and sent to the Resource Center. The first screen of the wild-type Cdc28 bait on -histidine media + 2mM 3-aminotriazole showed a somewhat high His+ background, but 13 positives were clearly visible above background, including three proteins known to interact with Cdc28.
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Modeling gene expression in yeast using large degenerate libraries
  • 批准号:
    10172925
  • 项目类别:
  • 资助金额:
    $35.09万
  • 财政年份:
    2018
  • 负责人:
    STANLEY FIELDS
  • 依托单位:
INTERROGATION OF E3 UBIQUITIN LIGASE CATALYSIS BY DEEP MUTATIONAL SCANNING
  • 批准号:
    8365800
  • 项目类别:
  • 资助金额:
    $2.18万
  • 财政年份:
    2011
  • 负责人:
    STANLEY FIELDS
  • 依托单位:
CHARACTERIZATION OF SMALL MOLECULE METABOLITES
  • 批准号:
    8365852
  • 项目类别:
  • 资助金额:
    $2.18万
  • 财政年份:
    2011
  • 负责人:
    STANLEY FIELDS
  • 依托单位:
A STRATEGY TO QUANTIFY PROTEIN STABILITY
  • 批准号:
    8365801
  • 项目类别:
  • 资助金额:
    $2.18万
  • 财政年份:
    2011
  • 负责人:
    STANLEY FIELDS
  • 依托单位:
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