WASTE SITE ASSESSMENT

描述：更新项目的目标是开发一个 评估与下列物质有关的人类健康风险的综合程序： a超级基金网站。生物学试验将提供数据， 免疫毒性、遗传毒性、发育和神经毒性潜力 环境样品。将进行五项实验， 该项目旨在评估基于生物学的协议的实用性 评估超级基金项目的人类健康风险前两 实验将评估与复杂混合物相关的风险， 超级基金场地以及模型化合物和重组混合物 AH和HAH化学品的代表。实验1设计 比较使用生物学方法或 化学分析这项实验将涉及收集 样本来自超级基金站点和匹配的对照区域。环境 样本将从范围广泛的超级基金地点收集 AH的代表（即炼油厂废物、煤气化废物），以及 有害健康物质（即木材防腐废物、受多氯联苯污染的土壤和沉积物） 污染物。研究中心样本的初始筛选将使用 一项急性毒性试验和两项微生物遗传毒性试验。选择 还将对样品进行定量GC/MS分析。基于 根据初步筛选的结果， 将在2010年开发的毒理学测试中进行分析。 项目1至6。将进行第二次实验， 从选定的复合物中分离出毒性最大的组分 混合物。分离的组分将复溶用于试验（如 单独的化合物和混合物），以评估 复杂混合物中各成分的潜在相互作用。数据 将用于提供生物测试数据 描述复杂混合物组分之间的相互作用， 潜在毒性当量因子（TEFs）。第三和第四 实验将侧重于监测复杂混合物的解毒情况。 这些实验将提供信息，以确定的能力， 减少毒性和对人类潜在威胁的补救技术 环境中复杂混合物的健康。在第五个实验中， 将对页硅酸盐粘土（天然和化学改性）进行评估 因为它们能够紧密结合并解毒目标化学物质。的 化学吸附的位点和分子机制将被阐明。

Description: The objective of the renewal project is to develop a comprehensive procedure to assess the human health risk associated with a Superfund site. Biological testing will provide data to define the immunotoxic, genotoxic, developmental and neurotoxic potential of environmental samples. Five experiments will be conducted as part of this project to evaluate the utility of a biologically-based protocol to estimate human health risk at a Superfund site. The first two experiments will assess the risk associated with complex mixtures from Superfund sites as well as model compounds and reconstituted mixtures representative of the AH and HAH chemicals. Experiment 1 is designed to compare estimates of human health risk using either biological or chemical analysis. This experiment will involve the collection of samples from Superfund sites and matched control areas. Environmental samples will be collected from a broad range of Superfund sites representative of AH (i.e. refinery waste, coal gasification waste) and HAH (i.e. wood-preserving waste, PCB contaminated soils and sediments) contaminants. An initial screen of site samples will be performed using an acute toxicity and two microbial genotoxicity assays. Selected samples will also be subjected to a quantitative GC/MS analysis. Based on the results of the preliminary screen, a limited number of samples will be analyzed in the battery of toxicological tests developed in Projects 1 through 6. A second experiment will be conducted to fractionate and isolate the most toxic components from selected complex mixtures. Isolated components will be reconstituted for testing (as individual compounds and mixtures) in the bioassays to evaluate the potential interactions of the components of a complex mixture. The data from these experiments will be used to provide biological test data describing the interactions of the components of complex mixtures and their potential toxic equivalency factors (TEFs). The third and fourth experiments will focus on monitoring detoxification of complex mixtures. These experiments will provide information to define the ability of remedial techniques to reduce toxicity and the potential threat to human health of complex mixtures in the environment. In the fifth experiment, phyllosilicate clays (natural and chemically-modified) will be evaluated for their abilities to tightly bind and detoxify target chemicals. The sites and molecular mechanisms of chemisorption will be elucidated.