Upgrade of Andor Dragonfly for Four Dimensional Cellular Medicine and Super-resolution microscopy

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Here we are applying for an upgrade for the Andor Dragonfly spinning disk confocal/ super-resolution laser scanning microscope. We Beta-tested the Andor Dragonfly in 2016/17 and our system was the first in Europe. In the past 6 years it has made a significant contribution to the publication of over 22 papers. It has been used so much (>1000 hours per annum) in the ensuing six years that the stand and sCMOS camera, built around a Nikon microscope stand requires replacement. The system has carried out over 6000 hours of research in the past 6 years and is the busiest machine in the advanced imaging core facility. Since Dragonfly's original installation both Andor and Nikon have released new products that make the new Dragonfly system an even better fit to our research. We have been gifted an unused Nikon Ti2 stand which needs a Hardware autofocus, a laser and new objectives to be fitted. An automatic water dispenser, new silicone immersion objective and new highly sensitive camera (SoNa) will provide unparalleled depth of field and field of view to facilitate high resolution live imaging of organoids, model organisms and organotypics. To ensure that the system is well supported it will be housed in a dedicated live imaging suite and supported by expert technicians. We will keep the system under an all inclusive service contract. We are confident with the upgrades the Dragonfly will continue to be the cornerstone of our MRC HGU research. The Dragonfly is open access as part of the Edinburgh Super-resolution Consortium (ESRIC).