Andor Dragonfly 202 Spinning Disk Confocal Microscope for the University of North Carolina at Chapel Hill

项目总结/摘要 位于查佩尔山的北卡罗来纳州大学（UNC-CH）正在申请Andor Dragonfly 202旋转盘 共聚焦显微镜。这款显微镜非常适合对活体样品进行灵敏、快速的成像。它将使研究 在干细胞生物学，神经科学，癌症生物学，植物科学和细胞生物学，在17个实验室，从10 UNC-CH三所学校的部门。这种显微镜将允许目前不可能的实验 在UNC-CH，由于没有具备Dragonfly的灵敏度、大视场 在厚组织中的性能，以及与广泛的荧光团的兼容性。该补助金详细概述了 UNC-CH目前的成像选择如何不足，为Dragonfly 202是最好的提供了证据 在所有考虑的系统中选择，并证明了使关键 实验可能。简言之，所要求的系统将有6条激光线，覆盖所有常用的 荧光团（BFP，CFP，GFP，YFP，RFP，远红），灵敏快速的sCMOS相机，EMCCD 具有最高灵敏度的摄像头，适合细胞级或亚细胞级细节的物镜，以及 培养箱外壳，以确保活样品的活力，用于多日实验。学校要求 联邦基金474，445美元，将补充内部来源的76，455美元，以确保该工具 具有支持UNC-CH研究人员需求所需的所有功能。 该显微镜将被放置在显微镜服务实验室，这是一个充电核心设施，具有30年以上的寿命。 支持UNC-CH社区显微镜需求的历史。如本申请中所详述，核心是 在大学的大力支持下，拥有在仪器类型方面具有专业知识的人员， 要求，一个强大的跟踪记录培训和协助研究人员，以及一个庞大而广泛的用户群， 超过一百个实验室和几百名研究人员。 我们预计这台仪器将迅速成为UNC-CH活细胞成像的主力，并将催化 多个学科领域的重大发现。

Project Summary/Abstract The University of North Carolina at Chapel Hill (UNC-CH) is requesting an Andor Dragonfly 202 Spinning Disk Confocal Microscope. This microscope is ideal for sensitive, fast imaging of live samples. It will enable research in Stem Cell Biology, Neuroscience, Cancer Biology, Plant Science and Cell Biology, in 17 laboratories from 10 Departments across 3 Schools at UNC-CH. This microscope will allow experiments that are currently not possible at UNC-CH as there are no available systems with the Dragonfly’s combination of sensitivity, large field of view, performance in thick tissue, and compatibility with a broad palette of fluorophores. This grant outlines in detail how the current imaging options at UNC-CH fall short, provides evidence for why the Dragonfly 202 is the best option among all the systems considered and justifies specific features that are required to make critical experiments possible. Briefly, the requested system will have 6 laser lines that cover all commonly-used fluorophores (BFP, CFP, GFP, YFP, RFP, far red), a sCMOS camera that is sensitive and fast, an EMCCD camera with maximum sensitivity, objectives ideal for either cellular-level or subcellular-level details, and an incubator enclosure to ensure viability of live samples for multi-day experiments. The University is requesting $474,445 in Federal Funds, which it will supplement with $76,455 from internal sources to ensure the instrument has all the features needed to support UNC-CH researchers’ needs. This microscope will be placed in the Microscopy Services Laboratory, a recharge core facility with a 30+ year history supporting the microscopy needs of the UNC-CH community. As detailed in this application, the core is strongly supported by the University, has personnel with demonstrated expertise in the type of instrument being requested, a strong track record training and assisting researchers, and a large and broad user base of more than a hundred labs and several hundred researchers. We expect this instrument will rapidly become a workhorse for live-cell imaging at UNC-CH and will catalyze major discoveries across multiple subject areas.