Andor Dragonfly 202 Spinning Disk Confocal Microscope for the University of North Carolina at Chapel Hill

项目摘要/摘要 北卡罗来纳大学教堂山分校(UNC-CH)正在申请Andor蜻蜓202旋转磁盘 共焦显微镜。这款显微镜非常适合对活体样品进行灵敏、快速的成像。它将使研究成为可能 干细胞生物学、神经科学、癌症生物学、植物科学和细胞生物学，来自10个实验室的17个实验室 北卡罗来纳州大学的3所学校的系。这台显微镜将允许进行目前不可能进行的实验 在北卡罗来纳大学，由于没有蜻蜓的敏感度、大视场、 在厚组织中的性能，以及与广泛的荧光团调色板的兼容性。这项拨款详细列出了 UNC-CH目前的成像选项如何不足，为为什么蜻蜓202是最好的提供了证据 所有考虑过的系统中的选项，并证明关键功能所需的特定功能是合理的 实验是可能的。简而言之，所要求的系统将有6条激光线路，覆盖所有常用的 荧光团(BFP、CFP、GFP、YFP、RFP、远红)，灵敏快速的sCMOS相机，EMCCD 具有最高灵敏度的摄像头、适合于细胞级别或亚细胞级别细节的目标，以及 保温箱外壳，以确保活体样品的生存能力，以进行多天的实验。这所大学要求 474,445美元的联邦资金，它将从内部来源补充76,455美元，以确保该工具 具有支持北卡罗来纳大学-CH研究人员需求的所有功能。 这台显微镜将放置在显微镜服务实验室，这是一个可充电30年的核心设施 支持北卡罗来纳大学-CH社区显微镜需求的历史。正如本应用程序中详细描述的那样，核心是 得到大学的大力支持，拥有在以下类型的仪器方面表现出的专业知识的人员 要求，强大的记录培训和协助研究人员，以及更多的大量和广泛的用户基础 超过一百个实验室和数百名研究人员。 我们预计这台仪器将迅速成为北卡罗来纳大学的活细胞成像的主力，并将催化 多个学科领域的重大发现。

Project Summary/Abstract The University of North Carolina at Chapel Hill (UNC-CH) is requesting an Andor Dragonfly 202 Spinning Disk Confocal Microscope. This microscope is ideal for sensitive, fast imaging of live samples. It will enable research in Stem Cell Biology, Neuroscience, Cancer Biology, Plant Science and Cell Biology, in 17 laboratories from 10 Departments across 3 Schools at UNC-CH. This microscope will allow experiments that are currently not possible at UNC-CH as there are no available systems with the Dragonfly’s combination of sensitivity, large field of view, performance in thick tissue, and compatibility with a broad palette of fluorophores. This grant outlines in detail how the current imaging options at UNC-CH fall short, provides evidence for why the Dragonfly 202 is the best option among all the systems considered and justifies specific features that are required to make critical experiments possible. Briefly, the requested system will have 6 laser lines that cover all commonly-used fluorophores (BFP, CFP, GFP, YFP, RFP, far red), a sCMOS camera that is sensitive and fast, an EMCCD camera with maximum sensitivity, objectives ideal for either cellular-level or subcellular-level details, and an incubator enclosure to ensure viability of live samples for multi-day experiments. The University is requesting $474,445 in Federal Funds, which it will supplement with $76,455 from internal sources to ensure the instrument has all the features needed to support UNC-CH researchers’ needs. This microscope will be placed in the Microscopy Services Laboratory, a recharge core facility with a 30+ year history supporting the microscopy needs of the UNC-CH community. As detailed in this application, the core is strongly supported by the University, has personnel with demonstrated expertise in the type of instrument being requested, a strong track record training and assisting researchers, and a large and broad user base of more than a hundred labs and several hundred researchers. We expect this instrument will rapidly become a workhorse for live-cell imaging at UNC-CH and will catalyze major discoveries across multiple subject areas.