Andor Revolution XD Live Cell Imaging System with Photomanipulation Laser

描述（由申请人提供）：本申请旨在申请资金购买Andor Revolution XD活细胞成像系统，该系统配备了旋转圆盘共聚焦显微镜和FRAPPA光操纵激光模块，以进行关键的光消融、光活化、光转化。本申请中提出的系统设置是量身定制的，在大量使用的成像核心设施中具有高度通用性，具有多种应用，并将充分利用Andor旋转盘共焦系统提供的独特功能。该系统将（1）配备 FRAPPA激光模块对许多光操纵实验至关重要;以及（2） 将安装在Nikon PerfectFocus Ti倒置显微镜上，以便在长期成像期间进行严格的焦平面补偿。具体而言，该系统被配置为通过同时满足以下应用的需求来满足在大量使用的细胞成像设备中遇到的广泛需求：（i）以每秒> 5幅图像的帧速率快速获取高质量图像，（ii）最小的成像相关光漂白，（iii）用于非常长期的活细胞成像的稳定阶段，（v）用于光操纵实验如FRAP、FLIP、FRET、光转化和光活化的外部激光器。我们在这里提出的初步数据的应用程序，我们建议执行的系统，从而提供重要的证据，这确实是系统，将提供所有的工具，我们预期和需要的好处。

DESCRIPTION (provided by applicant): This application is to request funds to purchase the Andor Revolution XD live cell imaging system equipped with spinning disk confocal microscope and the FRAPPA photomanipulation laser module to perform the crucial photoablation, photoactivation, photoconversion. The system set-up proposed in this request is tailor-designed to be highly versatile in a heavily used imaging core facility with a multitude of applications and will take full advantage of the unique features afforded by the Andor spinning disk confocal system. The system will be (1) equipped with the FRAPPA laser module critical for numerous photomanipulation experiments; and (2) will be fitted on Nikon PerfectFocus Ti inverted microscope for rigorous focal plane compensation during long-term imaging. Specifically, this system is configured to cater to the wide-ranging need encountered in the heavily used Cell Imaging Facility by simultaneously meeting the demand of the following applications: (i) fast acquisition of high quality image at > 5 images per second frame rate, (ii) minimal imaging-related photobleaching, (iii) stable stage for very long term live cell imaging, (iv) live cell stage-top incubation chamber (v) external lasers for photomanipulation experiments such as FRAP, FLIP, FRET, photoconversion and photoactivation. We present here preliminary data for the applications we propose to perform on the system, thus provide important evidence that this is indeed the system that will deliver all the instrumental benefits we anticipate and need.