Andor Revolution XD Live Cell Imaging System with Photomanipulation Laser

描述（由申请人提供）：本申请申请资金用于购买Andor Revolution XD活细胞成像系统，该系统配备旋转盘共聚焦显微镜和FRAPPA光电操作激光模块，用于进行关键的光烧蚀、光活化、光转换。该请求中提出的系统设置是量身定制的，在大量使用的成像核心设施中具有高度通用性，具有多种应用，并将充分利用Andor旋转盘共聚焦系统提供的独特功能。该系统将配备

DESCRIPTION (provided by applicant): This application is to request funds to purchase the Andor Revolution XD live cell imaging system equipped with spinning disk confocal microscope and the FRAPPA photomanipulation laser module to perform the crucial photoablation, photoactivation, photoconversion. The system set-up proposed in this request is tailor-designed to be highly versatile in a heavily used imaging core facility with a multitude of applications and will take full advantage of the unique features afforded by the Andor spinning disk confocal system. The system will be (1) equipped with the FRAPPA laser module critical for numerous photomanipulation experiments; and (2) will be fitted on Nikon PerfectFocus Ti inverted microscope for rigorous focal plane compensation during long-term imaging. Specifically, this system is configured to cater to the wide-ranging need encountered in the heavily used Cell Imaging Facility by simultaneously meeting the demand of the following applications: (i) fast acquisition of high quality image at > 5 images per second frame rate, (ii) minimal imaging-related photobleaching, (iii) stable stage for very long term live cell imaging, (iv) live cell stage-top incubation chamber (v) external lasers for photomanipulation experiments such as FRAP, FLIP, FRET, photoconversion and photoactivation. We present here preliminary data for the applications we propose to perform on the system, thus provide important evidence that this is indeed the system that will deliver all the instrumental benefits we anticipate and need.