Multiplex biomarker assay reader- Purchase of Electrochemiluminescence Reader under the MRC Equipment bid

多重生物标志物测定读数仪 - 根据 MRC 设备投标购买电化学发光读数仪

• 批准号: MR/X012492/1
• 负责人: Sara Wells
• 金额: $ 16.89万
• 依托单位: MRC Harwell Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012492%2F1
• 关键词: Multiplex; biomarker; assay; reader; Purchase

The Mary Lyon Centre is a large-scale mouse genetics infrastructure with activities focused on applying mouse genetics, mutagenesis and phenotypic screening to investigate mammalian gene function at a systems level and in disease-states. Our work is aimed at generating new animal models, which shedding light on key aspects of human disease. One of the key challenges in the use of animal models is relevance to human disease. Whilst genetic alterations found in human patients can be introduced into mouse strains, analysis of how the disease manifest and progresses needs to align closely with patient data to ensure the model will be instructive for further research. High performance electrochemiluminescence (ECL) is a powerful, highly sensitive and selective tool in analytical applications. The QuickPex reader utilises this technology to generate light when stimulated by electricity to allow us to measure multiple key proteins and molecules simultaneously in individual samples. The combination of speed of analysis and the ability to multiplex immunoassays allows for increased throughput and productivity. Utilising the wide menu of commercially available mouse assays alongside the ability to develop specific assays for targeted research allows for highly translatable and sensitive results that will provide accurate preclinical models for the study of human disease. We are especially invested in the research of cancer, the microbiome, immune responses, and mitochondrial diseases and the ability to use, or customised panels that are targeted towards inflammation, toxicity and metabolism will be invaluable in this research.

玛丽·里昂中心是一个大规模的小鼠遗传学基础设施，其活动重点是应用小鼠遗传学、突变和表型筛选在系统水平和疾病状态下研究哺乳动物的基因功能。我们的工作旨在建立新的动物模型，从而揭示人类疾病的关键方面。使用动物模型的关键挑战之一是与人类疾病的相关性。虽然在人类患者中发现的基因改变可以被引入老鼠品系，但对疾病如何表现和进展的分析需要与患者数据密切一致，以确保该模型将对进一步的研究具有指导意义。高性能电致化学发光(ECL)是一种功能强大、灵敏度高、选择性强的分析工具。QuickPex阅读器利用这项技术在受到电刺激时产生光，使我们能够同时测量单个样本中的多个关键蛋白质和分子。分析速度和多重免疫分析的能力相结合，可以提高产量和生产率。利用广泛的商业可获得的小鼠检测菜单，以及为靶向研究开发特定检测的能力，可以获得高度可翻译和敏感的结果，将为人类疾病研究提供准确的临床前模型。我们特别投资于癌症、微生物组、免疫反应和线粒体疾病的研究，使用或针对炎症、毒性和新陈代谢的定制面板的能力在这项研究中将是无价的。