Translational Photonic Fingerprinting- STELLARIS FALCON FLIM CONFOCAL

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Researchers working at Edinburgh BioQuarter are heavily dependent on the imaging equipment centrally provided by the Confocal Advanced Light Microscopy (CALM) Facility. The existing CLSMs which are now 11 years old, have started to fail and are economically unsustainable. This has negatively impacted on the ongoing imaging work of many investigators. This platform needs to be urgently replaced and concurrently upgraded with an excitation light source, tuneable over a wide range of wavelengths, and fluorescence lifetime measurement (FLIM) capacity. Both of these capabilities are not available on the Campus, but are now essential for progressing optical molecular imaging platforms. Optical imaging probes are now being developed for clinical use with fibre-optic platforms for molecular characterisation and "fingerprinting" of pathological and physiological processes in clinical experimental studies. This suite of technology is now primed for translational development but a major obstacle is the lack of a suitable CLSM platform to enable screening, assessment and optimisation of imaging parameters prior to in-human translation.Across both the campus and the wider university and academic and industrial collaborators , FLIM is an emerging and core technology to help discover key pathways in cells and tissues.The platform is essential to fully exploit interdisciplinary research in Edinburgh supported by large scale programmes which are gaining insight into key processes in inflammation and host-pathogen interactions.