Translational Photonic Fingerprinting- STELLARIS FALCON FLIM CONFOCAL

平移光子指纹识别 - STELLARIS FALCON FLIM CONFOCAL

• 批准号: MR/X013499/1
• 负责人: Kev Dhaliwal
• 金额: $ 50.77万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013499%2F1
• 关键词: Translational; Photonic; Fingerprinting; STELLARIS; FALCON

Researchers working at Edinburgh BioQuarter are heavily dependent on the imaging equipment centrally provided by the Confocal Advanced Light Microscopy (CALM) Facility. The existing CLSMs which are now 11 years old, have started to fail and are economically unsustainable. This has negatively impacted on the ongoing imaging work of many investigators. This platform needs to be urgently replaced and concurrently upgraded with an excitation light source, tuneable over a wide range of wavelengths, and fluorescence lifetime measurement (FLIM) capacity. Both of these capabilities are not available on the Campus, but are now essential for progressing optical molecular imaging platforms. Optical imaging probes are now being developed for clinical use with fibre-optic platforms for molecular characterisation and "fingerprinting" of pathological and physiological processes in clinical experimental studies. This suite of technology is now primed for translational development but a major obstacle is the lack of a suitable CLSM platform to enable screening, assessment and optimisation of imaging parameters prior to in-human translation.Across both the campus and the wider university and academic and industrial collaborators , FLIM is an emerging and core technology to help discover key pathways in cells and tissues.The platform is essential to fully exploit interdisciplinary research in Edinburgh supported by large scale programmes which are gaining insight into key processes in inflammation and host-pathogen interactions.

在爱丁堡生物区工作的研究人员严重依赖共聚焦高级光学显微镜（CALM）设施集中提供的成像设备。现有的CLSM已经有11年的历史，已经开始失败，在经济上是不可持续的。这对许多研究人员正在进行的成像工作产生了负面影响。该平台需要紧急更换，同时升级为激发光源，可在宽波长范围内调谐，并具有荧光寿命测量（FLIM）能力。这两种能力在校园里都没有，但现在对于发展光学分子成像平台至关重要。目前正在开发光学成像探针，用于临床使用，光纤平台用于临床实验研究中病理和生理过程的分子表征和“指纹”。这套技术现在已经准备好进行转化开发，但一个主要障碍是缺乏合适的CLSM平台，无法在人体翻译之前对成像参数进行筛选、评估和优化。在校园和更广泛的大学以及学术和工业合作者中，FLIM是一种新兴的核心技术，有助于发现细胞和组织中的关键通路。该平台对于充分利用爱丁堡的跨学科研究至关重要，大规模的项目正在深入了解炎症和宿主-病原体相互作用的关键过程。