Next Generation Flow Cytometry: Establishing Spectral Flow Cytometry to Revolutionise High Dimensional Analysis in Animal Models and Patient Cohorts

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Our bodies are composed of lots of different types of cells that perform different functions. If these cells don't interact properly this can lead to disease. Understanding how cell interactions go wrong is important to understand all human diseases. The purpose of this application is to buy a new machine, a spectral flow cytometer, that allows us to look at how lots of cells are acting simultaneously. This machine will be accessible to all researchers at The University of Manchester through the Flow Cytometry Facility. A spectral flow cytometer is a new type of technology that allows more characteristics and functions of each cell to be measured at the same time than was previously possible (67 characteristics/functions). Individual cells from the blood or from tissues, such as the lung, run through the flow cytometer suspended in liquid very rapidly allowing for information on millions of cells to acquired in a matter of minutes. Researchers across the University will use the machine to understand how cells go wrong in inflammatory diseases and infections and how drugs might be used to target specific immune cell populations.