Next Generation Flow Cytometry: Establishing Spectral Flow Cytometry to Revolutionise High Dimensional Analysis in Animal Models and Patient Cohorts

下一代流式细胞术：建立光谱流式细胞术彻底改变动物模型和患者队列的高维分析

• 批准号: MR/X013642/1
• 负责人: John Grainger
• 金额: $ 39.36万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013642%2F1
• 关键词: Next; Generation; Flow; Cytometry; Establishing

Our bodies are composed of lots of different types of cells that perform different functions. If these cells don't interact properly this can lead to disease. Understanding how cell interactions go wrong is important to understand all human diseases. The purpose of this application is to buy a new machine, a spectral flow cytometer, that allows us to look at how lots of cells are acting simultaneously. This machine will be accessible to all researchers at The University of Manchester through the Flow Cytometry Facility. A spectral flow cytometer is a new type of technology that allows more characteristics and functions of each cell to be measured at the same time than was previously possible (67 characteristics/functions). Individual cells from the blood or from tissues, such as the lung, run through the flow cytometer suspended in liquid very rapidly allowing for information on millions of cells to acquired in a matter of minutes. Researchers across the University will use the machine to understand how cells go wrong in inflammatory diseases and infections and how drugs might be used to target specific immune cell populations.

我们的身体由许多不同类型的细胞组成，它们执行不同的功能。如果这些细胞不能正常相互作用，就会导致疾病。了解细胞相互作用如何出错对于了解所有人类疾病非常重要。此应用程序的目的是购买一台新机器，即光谱流式细胞仪，它使我们能够观察有多少细胞同时起作用。曼彻斯特大学的所有研究人员都可以通过流式细胞仪设施使用这台机器。光谱流式细胞仪是一种新型技术，与以前相比，它可以同时测量每个细胞更多的特征和功能（67 个特征/功能）。来自血液或组织（例如肺）的单个细胞非常快速地通过悬浮在液体中的流式细胞仪，从而可以在几分钟内获取数百万个细胞的信息。大学的研究人员将使用该机器来了解细胞在炎症性疾病和感染中如何出错，以及如何使用药物来针对特定的免疫细胞群。