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INTERNEURON FUNCTION IN THE ZEBRAFISH SPINAL CORD

斑马鱼脊髓的中间神经元功能

基本信息

批准号：
6185631
负责人：
MELINA E HALE
金额：
$ 3.75万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-06 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6185631
关键词：
alternatives to animals in research behavioral /social science research tag chordate locomotion confocal scanning microscopy escape reaction ethology interneurons larva motor neurons reticulospinal tract spinal cord zebrafish

项目摘要

The objective of this project is to examine the structure and function of interneurons in the reticulospinal system controlling escape behavior in fishes. The neural circuit which triggers escape behavior is simple, involving relatively few cell types and cell numbers, making this an appropriate system to use as a case study in the reticulospinal control of locomotion. Several major goals of this project are to determine the neural components of the reticulospinal system for escape in zebrafish and to examine the functions of several classes of interneuron, commissural interneurons and descending interneurons, during the escape behavior. Cell ablation and in vivo calcium imaging with confocal microscopy will be used to address these goals. Larval zebrafish will be used as a model system for this study. They are ideal due to the near transparency of their bodies and the wealth of knowledge available on all aspects of their biology. This project will provide basic information on zebrafish neurobiology and on the roles of interneurons in reticulospinal systems.

本项目的目标是检查结构和功能网状脊髓系统中控制逃逸的中间神经元鱼的行为。触发逃避行为的神经回路是简单的，涉及相对较少的细胞类型和细胞数量，这是一个合适的系统，可用作网状脊髓控制运动本项目的几个主要目标是为了确定网状脊髓系统的神经成分并研究了几种蛋白质的功能，中间神经元、连合中间神经元和下行神经元的分类中间神经元，在逃避行为中。细胞消融和体内钙成像与共聚焦显微镜将用于解决这些目标。斑马鱼幼体将被用作这方面的模型系统 study.他们是理想的，由于近透明的机构以及丰富的生物学知识。这个项目将提供斑马鱼神经生物学的基本信息以及中间神经元在网状脊髓系统中的作用。