ASSAYS FOR ROS INDUCED DNA DAMAGE

ROS 引起的 DNA 损伤的测定

• 批准号: 2777546
• 负责人: HAROLD C BOX
• 金额: $ 10万
• 依托单位: ZEPTOMETRIX CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-08 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2777546
• 关键词: DNA damage; bioassay; chemical synthesis; formamide; free radical oxygen; high performance liquid chromatography; nuclease; technology /technique development; thin layer chromatography; thymine

DESCRIPTION: (adapted from applicant's abstract) The objective of the research is to develop highly sensitive, quantitative and facile assays for measuring DNA damage induced by reactive oxygen species (ROS). ROS have been implicated in aging, cancer and several neurodegenerative diseases. Initially three specific ROS-induced DNA lesions will be of particular interest, namely, the formamido remnant of pyrimidine bases, the thymine glycol lesion and the 6-hydroxy-5, 6-dihydrothymine lesion. These lesions will be measured in the form of modified dinucleosides obtained from enzymatic digests of DNA. The method of detection will be by 32P-post labeling. The chief technological component of the undertaking will be the synthesis of modified dimers for use as carriers and internal standards. These carriers are necessary for selectivity and unequivocal identification of the lesions of interest. The internal standards are required for quantification of the lesions. These materials will be offered in kits that should make the detection of ROS-induced DNA damage convenient, sensitive and straightforward. It is anticipated that the availability of these kits will facilitate research on the relationship between ROS and disease. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人的摘要）研究的目的 是为了开发高度敏感，定量和轻松的测定 活性氧（ROS）诱导的DNA损伤。 ROS已被牵连 在衰老，癌症和几种神经退行性疾病中。最初三个 特异性ROS诱导的DNA病变将特别感兴趣，即 嘧啶碱的甲米多残留物，胸腺甘氨酸病变和 6-羟基-5，6-二氢噻吩蛋白病变。这些病变将在 从DNA的酶促消化获得的改性二核苷的形式。这 检测方法将通过32p-post标记。主要技术 企业的组成部分将是修改的二聚体的合成 作为载体和内部标准。这些载体是必要的 对感兴趣病变的选择性和明确的鉴定。这 量化病变需要内部标准。这些 材料将以应检测ROS诱导的套件提供 DNA损伤方便，敏感且直接。预计 这些套件的可用性将促进有关关系的研究 在ROS和疾病之间。 拟议的商业应用程序：不可用