ASSAYS FOR ROS INDUCED DNA DAMAGE

ROS 引起的 DNA 损伤的测定

• 批准号: 2777546
• 负责人: HAROLD C BOX
• 金额: $ 10万
• 依托单位: ZEPTOMETRIX CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-08 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2777546
• 关键词: DNA damage; bioassay; chemical synthesis; formamide; free radical oxygen; high performance liquid chromatography; nuclease; technology /technique development; thin layer chromatography; thymine

DESCRIPTION: (adapted from applicant's abstract) The objective of the research is to develop highly sensitive, quantitative and facile assays for measuring DNA damage induced by reactive oxygen species (ROS). ROS have been implicated in aging, cancer and several neurodegenerative diseases. Initially three specific ROS-induced DNA lesions will be of particular interest, namely, the formamido remnant of pyrimidine bases, the thymine glycol lesion and the 6-hydroxy-5, 6-dihydrothymine lesion. These lesions will be measured in the form of modified dinucleosides obtained from enzymatic digests of DNA. The method of detection will be by 32P-post labeling. The chief technological component of the undertaking will be the synthesis of modified dimers for use as carriers and internal standards. These carriers are necessary for selectivity and unequivocal identification of the lesions of interest. The internal standards are required for quantification of the lesions. These materials will be offered in kits that should make the detection of ROS-induced DNA damage convenient, sensitive and straightforward. It is anticipated that the availability of these kits will facilitate research on the relationship between ROS and disease. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(摘自申请者的摘要)研究的目的 是开发高度灵敏、定量和简便的检测方法 活性氧(ROS)引起的DNA损伤。ROS已被牵连 在衰老、癌症和几种神经退行性疾病方面。最初有三个 ROS诱导的特定DNA损伤将是特别感兴趣的，即 嘧啶碱甲酰胺残留物、胸腺嘧啶二醇损伤和 6-羟基-5，6-二氢胸腺嘧啶损伤。这些损伤将在 从DNA的酶消化中获得的修饰二核苷的形式。这个 检测方法将通过32P-POST标记。首席技术人员 该项目的组成部分将是合成供使用的改性二聚体 作为载体和内部标准。这些承运商对于 对感兴趣的病变进行选择性和明确的识别。这个 需要内部标准来对损伤进行量化。这些 材料将在试剂盒中提供，这些试剂盒应能够检测到ROS诱导的 DNA损伤检测方便、灵敏、直观。预计 这些试剂盒的提供将有助于对这种关系的研究 在ROS和疾病之间。 建议的商业应用：不可用