ASSAYS FOR ROS INDUCED DNA DAMAGE

ROS 引起的 DNA 损伤的测定

• 批准号: 2777546
• 负责人: HAROLD C BOX
• 金额: $ 10万
• 依托单位: ZEPTOMETRIX CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-08 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2777546
• 关键词: DNA damage; bioassay; chemical synthesis; formamide; free radical oxygen; high performance liquid chromatography; nuclease; technology /technique development; thin layer chromatography; thymine

DESCRIPTION: (adapted from applicant's abstract) The objective of the research is to develop highly sensitive, quantitative and facile assays for measuring DNA damage induced by reactive oxygen species (ROS). ROS have been implicated in aging, cancer and several neurodegenerative diseases. Initially three specific ROS-induced DNA lesions will be of particular interest, namely, the formamido remnant of pyrimidine bases, the thymine glycol lesion and the 6-hydroxy-5, 6-dihydrothymine lesion. These lesions will be measured in the form of modified dinucleosides obtained from enzymatic digests of DNA. The method of detection will be by 32P-post labeling. The chief technological component of the undertaking will be the synthesis of modified dimers for use as carriers and internal standards. These carriers are necessary for selectivity and unequivocal identification of the lesions of interest. The internal standards are required for quantification of the lesions. These materials will be offered in kits that should make the detection of ROS-induced DNA damage convenient, sensitive and straightforward. It is anticipated that the availability of these kits will facilitate research on the relationship between ROS and disease. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人摘要）研究目的 是开发高灵敏度，定量和简便的测定方法， 活性氧（ROS）引起的DNA损伤。ROS与 衰老、癌症和几种神经退行性疾病。最初三 特定的ROS诱导的DNA损伤将是特别感兴趣的，即， 嘧啶碱基的甲酰胺残基，胸腺嘧啶乙二醇损伤和 6-羟基-5，6-二氢胸腺嘧啶病变。这些病变将在 从DNA的酶解酶中获得的修饰的二核苷形式。的 检测方法为32 P-后标记。首席技术官 这项工作的一部分将是合成修饰的二聚体， 作为载体和内标。这些载体对于 选择性和明确识别感兴趣的病变。的 需要内标物来量化损伤。这些 材料将在试剂盒中提供，这些试剂盒应能够检测ROS诱导的 DNA损伤方便、灵敏、直接。预计各国 这些工具包的可用性将有助于研究这种关系， ROS和疾病之间的联系 拟议商业应用：不可用