FINNIGAN LCQTM BENCHTOP MS SYSTEM FOR HPLC

用于 HPLC 的 FINNIGAN LCQTM 台式 MS 系统

• 批准号: 2791778
• 负责人: HAROLD C BOX
• 金额: $ 20.96万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791778
• 关键词: analytical chemistry; biomedical equipment; biomedical equipment purchase; electrospray ionization mass spectrometry; gas chromatography mass spectrometry; high performance liquid chromatography

This proposal requests support for the purchase of an LC/MS system to augment the capabilities of the Mass Spectrometry Facility at Roswell Park Cancer Institute. The instrument requested is a Finnigan LCQ/TM ion trap benchtop mass spectrometer with electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources. Funds are also sought for a matching HPLC system, P4000-040 quaternary gradient system with UV/VIS detector and membrane degasser (from Thermo Separation Products, NJ) and a nanoelectrospray ion source (Protein Analysis Company, Odense, Denmark). The Finnigan LCQ/TM system has a 0-2000 dalton mass range analyzer and LC/MS/MS/n capabilities. The system is controlled by a PC with Microsoft Windows NT operating system. The biochemistry software is used for molecular weight determination from multi-charged ion envelope operated by ESI, peptide mapping of proteolytic digests and peptide sequence prediction and conformation. The Finnigan LCQ/TM LC/MS system will provide much needed analytical services to eleven NIH-funded investigators and two NIH funded research groups. It will be used to characterize proteins and peptide by their molecular weight, amino acid sequence, disulfide linkages and covalent and other modifications. This system will also be used to study oligonucleotides, oligosaccharides, drug metabolites, nucleic acid metabolites, etc. The LCQ/TM system will permit qualitative and quantitative studies using LC/MS, a technique not currently available at RPCI. LC/MS using selected ion monitoring via MS/MS techniques will be important for on-line quantitation of drug metabolites, oxidatively damaged oligonucleotides, nucleic acid metabolites, etc., at very low levels in complex biological matrices.

该提案要求支持购买LC/MS系统，以增强罗斯威尔公园癌症研究所的质谱设施的功能。所请求的仪器是带有电喷雾（ESI）和大气压力化学电离（APCI）源的Finnigan LCQ/TM离子陷阱台式质谱仪。还寻求匹配的HPLC系统，P4000-040 Quaternary梯度系统，带有UV/VIS探测器和膜DeGasser（来自Thermo Eaparation产品，NJ）和纳米电信离子离子源（蛋白质分析公司，Odense，Odense，丹麦）。 Finnigan LCQ/TM系统具有0-2000道尔顿质量范围分析仪和LC/MS/MS/N功能。该系统由带有Microsoft Windows NT操作系统的PC控制。生物化学软件用于从通过ESI操作的多电荷离子包膜，蛋白水解消化的肽映射和肽序列预测和构象的分子量测定。 Finnigan LCQ/TM LC/MS系统将为11名NIH资助的研究人员和两个NIH资助的研究小组提供急需的分析服务。它将通过其分子量，氨基酸序列，二硫键以及共价和其他修饰来表征蛋白质和肽。该系统还将用于研究寡核苷酸，寡糖，药物代谢产物，核酸代谢产物等。LCQ/TM系统将允许使用LC/MS进行定性和定量研究，这是当前在RPCI上可用的技术。使用MS/MS技术进行选定的离子监测的LC/MS对于在复杂的生物基质中非常低的水平，对于药物代谢产物，氧化损坏的寡核苷酸，核酸代谢物等的在线定量至关重要。