LC-MS/MS Assessments of Apoptosis/DNA Damage/Repair
细胞凋亡/DNA 损伤/修复的 LC-MS/MS 评估
基本信息
- 批准号:6815148
- 负责人:
- 金额:$ 14.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-07-01 至 2006-06-30
- 项目状态:已结题
- 来源:
- 关键词:DNA damageDNA repairX rayantineoplasticsapoptosisbleomycincarboplatincell linecisplatinclinical researchcyclophosphamidedoxorubicindrug screening /evaluationfemalefree radical oxygengemcitabinehigh performance liquid chromatographyhuman subjectliquid chromatography mass spectrometrylongitudinal human studyneoplasm /cancer chemotherapynucleic acid quantitation /detectionovary neoplasmstechnology /technique developmentultraviolet radiation
项目摘要
DESCRIPTION (provided by applicant): It is proposed that Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) be utilized to characterize the response of tumor cells to chemotherapy. Modern LC-MS/MS technology, employing an electrospray interface and triple quadrapole detection, provides unprecedented sensitivity, selectivity and the capability for measuring multiple products. It is proposed that LCMS/ MS technology be adapted to the measurement of DNA modifications induced by anticancer drugs including direct and/or indirect damage, various types of strand breaks and the products of apoptosis. Using a method of sample preparation that is highly effective in eliminating background it is feasible to measure simultaneously this variety of DNA lesions and modifications in a single sample. The feasibility of this approach is demonstrated in the application using LC-MS/MS measurements on cells exposed to cisplatin as an example. It is proposed that LC-MS/MS assay can be applied to cells obtained at surgery suing the culture methods presently employed to measure drug resisitivity. Differences in cell function revealed by LC-MS/MS assay will be complemented by array studies of gene expression. The first clinical application will be ovarian cancer tissue.
描述(申请人提供):建议使用LC-MS/MS(LC-MS/MS)来表征肿瘤细胞对化疗的反应。现代LC-MS/MS技术采用电喷雾界面和三重四极杆检测,提供了前所未有的灵敏度、选择性和测量多种产品的能力。建议将LC MS/MS技术应用于抗癌药物引起的DNA修饰,包括直接和/或间接损伤、各种类型的链断裂和细胞凋亡产物的检测。使用一种在消除背景方面非常有效的样品制备方法,在单个样品中同时测量这种不同的DNA损伤和修饰是可行的。以顺铂暴露细胞的LC-MS/MS测量为例,验证了该方法的可行性。建议LC-MS/MS法可应用于手术中获得的细胞,采用目前用于检测耐药性的培养方法。LC-MS/MS分析揭示的细胞功能差异将得到基因表达阵列研究的补充。第一个临床应用将是卵巢癌组织。
项目成果
期刊论文数量(0)
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{{ truncateString('HAROLD C BOX', 18)}}的其他基金
LC-MS/MS Assessments of Apoptosis/DNA Damage/Repair
细胞凋亡/DNA 损伤/修复的 LC-MS/MS 评估
- 批准号:
6914944 - 财政年份:2004
- 资助金额:
$ 14.8万 - 项目类别:
FINNIGAN LCQTM BENCHTOP MS SYSTEM FOR HPLC
用于 HPLC 的 FINNIGAN LCQTM 台式 MS 系统
- 批准号:
2791778 - 财政年份:1999
- 资助金额:
$ 14.8万 - 项目类别:
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