Dissemination of refinements in mouse experimental stroke models to the scientific community.

向科学界传播小鼠实验性中风模型的改进。

• 批准号: NC/R000883/1
• 负责人: Claire Gibson
• 金额: $ 8.31万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NC%2FR000883%2F1
• 关键词: Dissemination; refinements; mouse; experimental; stroke

In the UK, cerebral stroke is the 3rd cause of death, the leading cause of severe adult disability in adults and costs the NHS an estimated £3 billion per year. The generation of novel and rational therapeutics is essential. Thus, every year thousands of animals are used experimentally to model stroke and investigate potential therapies. Mice are commonly used in experimental studies aimed at modelling stroke not least because they are useful for genetic manipulation studies. However, studies using experimental stroke models tend to show a large variability in the data produced. This usually results in researchers using an increased number of animals per study in order to try and detect a significant difference. We proposed that a large portion of this variability may be due to differences in the anatomy of blood vessels in the brain and an alternative approach, which we have previously developed, reduces the variability seen. Our aim here is to disseminate this refined approach within the scientific community so that other labs performing experimental stroke studies will produce data with reduced variability and therefore reduce the number of animals used in such experiments.

在英国，脑卒中是第三大死因，是成年人严重残疾的主要原因，NHS每年估计花费30亿英镑。新的和合理的治疗方法的产生是必不可少的。因此，每年都有成千上万的动物被用于实验性中风模型和研究潜在的治疗方法。小鼠通常用于旨在模拟中风的实验研究，尤其是因为它们对遗传操作研究很有用。然而，使用实验性中风模型的研究往往显示出所产生的数据的较大变异性。这通常会导致研究人员在每次研究中使用更多的动物，以尝试检测显着差异。我们提出，这种变异性的很大一部分可能是由于大脑中血管解剖结构的差异，我们以前开发的另一种方法减少了所见的变异性。我们的目标是在科学界传播这种改进的方法，以便其他进行实验性中风研究的实验室将产生具有减少变异性的数据，从而减少此类实验中使用的动物数量。