Ex vivo model for the study of epicardium-targeted therapies

用于研究心外膜靶向治疗的离体模型

• 批准号: NC/R001006/1
• 负责人: Paola Campagnolo
• 金额: $ 24.59万
• 依托单位: University of Surrey
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NC%2FR001006%2F1
• 关键词: Ex; vivo; model; study; epicardium

The recovery capacity of the adult heart after injury is severely limited by the low number of regenerating cells within this tissue. The epicardium, the most external layer of the heart, contains cells able to home to the injured heart muscle and promote its recovery. The models available to the scientists to study this phenomenon and develop strategies aimed at facilitating and improving the reparative process are limited and mainly based on regulated procedures (surgeries) performed on animals. In this project we will develop a model based on leftover pig heart tissues to mimic in a dish the behaviour of the epicardium after stimulation.Pig hearts will be reduced to thin slices including both the epicardium and underlying heart muscle; the slices will be subjected to treatments to simulate the aftermath of heart disease and stimulated with known therapeutic substances to assess the capacity of our system to reproduce what has been observed in the animal models.Once our model is tested, it will be used to develop new therapies harnessing the reparative potential of the epicardium.

受伤后成人心脏的恢复能力受到该组织中再生细胞数量少的数量的严重限制。心脏心脏是心脏最外层的心脏，其中包含能够回家受伤的心肌并促进其恢复的细胞。科学家可用的模型研究这种现象并制定旨在促进和改善修复过程的策略是有限的，并且主要基于对动物进行的调节程序（手术）。在这个项目中，我们将开发一个基于剩余的猪心组织的模型，以模仿刺激后心外膜的行为。该切片将受到治疗，以模拟心脏病的后果，并用已知的治疗物质进行刺激，以评估我们系统的能力再现动物模型中观察到的内容。一旦测试了我们的模型，它将用于开发新的疗法来利用心肌的修复潜力。