EXPRESSION, STRUCTURE AND FUNCTION OF FILAGGRIN

丝聚蛋白的表达、结构和功能

• 批准号: 6160821
• 负责人: P STEINERT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6160821
• 关键词: apoptosis; cell differentiation; cytoskeletal proteins; gene expression; genetic promoter element; human tissue; intermediate filaments; keratin; keratinocyte; nuclear factor kappa beta; protein sequence; protein structure function; skin; transcription factor; transfection /expression vector

Filaggrin, the processed form of profilaggrin, is a major product of terminally differentiating mammalian epidermis. Based on in vitro experiments, filaggrin is thought to be involved in the aggregation and specific alignment of keratin intermediate filaments during the final stages of development in the epidermis in vivo. We have explored the regulation of expression of the profilaggrin gene and have characterized the proximal promoter. An AP1 site and its cognate binding c-fos and c-jun proteins confer keratinocyte-specific expression to the gene, in concert with neighboring Sp1, ets-like, and NF-kB elements. We have discovered a novel ets transcription protein that seems to function in the regulation of expression of several late differentiation genes in the epidermis, including profilaggrin.

聚丝蛋白是聚丝蛋白原的加工形式，是聚丝蛋白的主要产物。 哺乳动物表皮的终末分化。 基于体外 在实验中，丝聚蛋白被认为参与聚集， 角蛋白中间丝的特定排列 在体内表皮的发育阶段。我们已经探索了 调节聚丝蛋白原基因的表达，并已表征 近端启动子 AP 1位点及其同源结合c-fos和 c-jun蛋白赋予该基因角质细胞特异性表达， 与相邻的Sp1、类ETS和NF-κ B元素相协调。 我们有 发现了一种新的ets转录蛋白， 几个晚期分化基因的表达调控 表皮，包括聚丝蛋白原。