EXPRESSION, STRUCTURE AND FUNCTION OF LORICRIN, A MAJOR CELL ENVELOPE PROTEIN

主要细胞包膜蛋白 Loricrin 的表达、结构和功能

• 批准号: 3804561
• 负责人: P STEINERT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804561
• 关键词: crosslink; cytoskeletal proteins; dipeptides; gene expression; genetically modified animals; glycine; human tissue; keratinocyte; laboratory mouse; membrane proteins; messenger RNA; molecular cloning; pathologic keratinization; polymerase chain reaction; protein structure function; restriction fragment length polymorphism

The cell envelope constitutes a thick layer of highly insoluble protein on the inside of the plasma membrane of cornfield epidermal cells and of other stratified squamous epithelial cells. Of several putative protein components, none has hitherto proven to be a major component because of major differences in amino acid composition, levels of mRNA or of expressed protein. We have recently identified a new protein, termed loricrin, that fulfills all of the requirements of a major constituent of the cell envelope. Loricrin clones were first identified in a mouse epidermis, and now in more detail, from human epidermis. Loricrins are glycine-rich proteins that contain the highly flexible glycine loop motif. They are crosslinked in cell envelopes by isodipeptide Nepsilon- (gamma-glutamyl)lysine bonds. The possible role of loricrins in keratinizing disorders of epidermis is being investigated by transgenics, PCR, RFLP and genodermatoses analyses.

细胞包膜由一层高度不溶性的蛋白质构成