ADVERSE EFFECTS OF OXIDATIVE STRESS DURING PREGNANCY

工作总结：氧化损伤与病理学有关， 几种疾病，但直到最近才有人建议， 在不良生殖条件下的作用。 许多环境和 职业暴露是已知的促氧化剂（例如，许多溶剂和 金属），并且它们可能通过引起氧化而对生殖产生不利影响。 损害这个研究项目探讨了 环境暴露、氧化应激和不良生殖 条件 我们有两个主要目标。首先是对试点数据的分析 这项研究将解决抗- 氧化剂和氧化剂状态由于季节性和个体内变化 和储存程序，并比较结果，在怀孕和 非妊娠女性。迄今为止的调查结果表明， 储存超过几个小时而不改变含量 谷胱甘肽和脂质过氧化物显着。 第二个目标是工作 与实验室科学家一起设计了一项研究， 氧化状态是否适合流行病学研究。 这项工作是 去年秋天在NIEHS举行的一次国际会议的延续， 调查了氧化应激标志物领域。此外，分析 从保加利亚现有数据中抽取少量样本， 污染物、氧化损伤标志物和生殖结果 孕妇及其婴儿继续。

Summary of Work: Oxidative damage has been implicated in the pathology of several diseases, but only recently has it been suggested to have a role in adverse reproductive conditions. Many environmental and occupational exposures are known pro-oxidants (e.g., many solvents and metals), and they may adversely affect reproduction by causing oxidative damage. This research project explores the relationship between environmental exposures, oxidative stress, and adverse reproductive conditions. We working with two major aims. First is analysis of data from a pilot study that will resolve the amount of variability in the markers of anti- oxidant and oxidant status due to seasonal and intra-individual variation and to storage procedures, and to compare the results in pregnant and non-pregnant women. Findings to date indicate that samples cannot be stored at all for more than a few hours without altering the level of glutathione and lipid peroxides significantly. A second aim is to work with laboratory scientists in designing a study to evaluate mamkers of oxidative status for suitability in epidemiologic studies. This work is a continuation of an international meeting held last fall at NIEHS that surveyed the field of oxidative stress markers. In addition, the analysis of small samples of existing data from Bulgaria concerning industrial pollutants, markers of oxidative damage, and reproductive outcome in pregnant women and their infants continues.