GENES REGULATED BY ABUSED DRUGS

滥用药物通过生化作用导致行为的长期变化 这些机制在很大程度上是未知的。药物改变后的变化 大脑中特定基因的表达可以提供一个重要的窗口 可能导致上瘾的生化底物。进一步探索 在这方面，我们已经建立和发展了减法微分 显示技术已经允许识别数十个 与候选药物调控基因相对应的不同的cDNA 在大脑中表达。在本财年，我们加强了对 其表达受苯丙胺调控的候选基因， 可卡因和吗啡。对这些cDNA的序列分析表明， 它们分为三类：新的cDNA，前面描述的那些 仅作为序列，以及属于特定基因家族的那些 功能含义。

Abused drugs produce long-lasting changes in behaviors via biochemical mechanisms that are largely unknown. Drug-altered changes in expression of specific genes in the brain can provide a major window on possible biochemical substrates for addiction. To further explore this area, we have established and developed subtracted differential display techniques that have allowed identification of dozens of distinct cDNAs that correspond to candidate drug-regulated genes expressed in brain. During this FY, we have enhanced characterization of candidate genes whose expression is regulated by amphetamine, cocaine and morphine. Sequence analyses of these cDNAs reveal that they fall into three groups: novel cDNAs, those previously described as sequences only, and those that fall into specific gene families with functional implications.