STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
批准号:
6161886
负责人:
R W HARTLEY
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
Bacillus bacterial proteins chemical stability enzyme activity enzyme biosynthesis enzyme inhibitors enzyme mechanism enzyme structure esterase inhibitor exoribonucleases gene expression gene mutation genetic regulatory element intermolecular interaction microorganism metabolism molecular cloning nucleic acid sequence protein engineering protein folding protein structure function recombinant DNA
中文摘要
两种蛋白质,藤蔓酶,芽孢杆菌胞外核糖核酸酶
英文摘要
Two proteins, barnase, the extracellular ribonuclease of Bacillus
amyloliquefaciens, and barstar, its intracellular inhibitor, are used
as a model system for the study of protein folding and protein-protein
interactions. Barnase is one of an homologous group of ribonucleases
ocurring in both prokaryotes and eukaryotes. Recombinant DNA techniques
are being applied with three major aims: (1) to facilitate production
of wild type and mutant proteins; (2) to examine the structural and
contro sequences of the genes; and (3) to make specific changes in the
sequences to test theories of folding and to probe the barnase-barstar
interaction. Both proteins can now be obtained from recombinant genes
in E. coli where expression of barstar counters the lethal effect of
barnase expression. The structures of both proteins and their complex
are known. A new crystallographic solution of the structure at 1.5
angstroms resolution has provided new insights. For example, several
water molecules seen in the barnase-barstar interface can be seen
already bound to free barnase. Several barnase-barstar pairs having
complementary mutations in the interface, obtained by an in vivo
selective technique, have been crystallized and structures are immiment.
Barstar also inhibits a group of RNases from Streptomyces strains. These
enzymes are distantly related to arnase with a sequence identity of only
25%. Among themselves, identify ranges from 40% to 70%. The structures
of two, RNases Sa and St, are known from work on nonrecombinant
material. We now have the structure of a third, RNase Sa2, both alone
and in complex with barstar. Overlapping fragments of the gene for RNase
St, which is poorly inhibited by barstar, have been cloned, allowing us
to correct several errors in its published amino acid sequence. The gene
for Sa2 homolog of barstar has now been cloned and sequenced. This
protein inhibits all the Streptomyces enzymes but apparently not
barnase. The controllable lethality of the barnase gene has been used
to advantage in producing a selective general purpose plasmid cloning
vector
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STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:5201904
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:3839583
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:3875541
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUENFACIENS
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批准号:3940232
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUENFACIENS
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批准号:4689017
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUENFACIENS
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批准号:3917361
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUENFACIENS
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批准号:3964011
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:3854534
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:3776059
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:3753948
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
STUDY OF A RIBONUCLEASE AND ITS INHIBITOR FROM BACILLUS AMYLOLIQUEFACIENS
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批准号:2572771
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:R W HARTLEY
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依托单位:
海外基金