LONG TERM RECORDING FROM AFFERENT TASTE FIBERS

味觉传入纤维的长期记录

• 批准号: 6176783
• 负责人: Robert M Bradley
• 金额: $ 23万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-29 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176783
• 关键词: afferent nerve; bioengineering /biomedical engineering; biomedical equipment development; cell cycle; chorda tympani; electronic recording system; implant; laboratory rat; longitudinal animal study; microelectrodes; miniature biomedical equipment; nervous system regeneration; neurofilament; single cell analysis; taste buds; telemetry

Development of an implantable, multielectrode array has made it possible to record chronically, over periods of several weeks, from sensory fibers of taste nerves that innervate the tongue. In the current proposal, the electrode array will be used to systematically test hypotheses about neural discharge properties of peripheral taste fibers over long time frames that encompass numerous cycles of taste bud cell turnover, and synapse remodeling. In addition, the multielectrode recording device will be progressively refined to improve and expand its range of capabilities. The rat chorda tympani nerve will be dissected and implanted with the multielectrode array through which taste nerve fibers regenerate. Subsequently, recordings will be made from the same single fiber, or sets of fibers, for weeks. In Specific Aim 1, chronic recordings from single taste fibers of the chorda tympani nerve will be used to test the hypotheses that response characteristics of taste fibers, and receptive field properties, remain stable during cycles of taste bud cell turnover and synapse remodeling. In Specific Aim 2, simultaneous recordings from several individual fibers will be studied over weeks to test the hypothesis that there is a unique pattern of fiber activity for separate chemical qualities, and that the pattern remains stable over time. In Specific Aim 3, an implantable telemetry system will be used with the multielectrode array to record from chorda tympani fibers in awake rats and investigate the pattern of neural activity during feeding and drinking. The implantable, multielectrode recording array and its further refinement make possible tests of hypotheses about basic taste neurobiology that could not be resolved with previous neurophysiological techniques. Also, studies of taste fiber responses in behaving animals become feasible with the implantable device. Furthermore, continued refinement of, and experiments with, the implantable electrode array can lead to potential applications as a neural prosthesis in regeneration and restoration of sensory and motor function after trauma or disease.

一种可植入的多电极阵列技术的发展，使得长期记录支配舌头的味觉神经的感觉纤维，持续数周成为可能。在目前的提议中，电极阵列将用于系统地测试长时间框架内周围味觉纤维的神经放电特性的假设，这些假设包括味蕾细胞更新和突触重塑的许多周期。此外，多电极记录装置将逐步完善，以提高和扩大其能力范围。大鼠鼓室索神经将被解剖并植入多电极阵列，通过多电极阵列使味觉神经纤维再生。随后，用同一根纤维或几组纤维连续数周进行录音。在Specific Aim 1中，来自鼓室索神经单个味觉纤维的慢性记录将用于测试味觉纤维的反应特征和感受野特性在味蕾细胞更新和突触重塑周期中保持稳定的假设。在具体目标2中，将在数周内研究几个单独纤维的同时记录，以验证不同化学性质的纤维活动存在独特模式的假设，并且该模式随着时间的推移保持稳定。在具体目标3中，将使用一个植入式遥测系统与多电极阵列一起记录清醒大鼠的鼓室索纤维，并研究进食和饮水时的神经活动模式。这种可植入的多电极记录阵列及其进一步的改进，使以前的神经生理学技术无法解决的关于基本味觉神经生物学假设的测试成为可能。此外，有了植入式装置，对行为动物味觉纤维反应的研究也变得可行。此外，可植入电极阵列的持续改进和实验可能会导致作为神经假体的潜在应用，用于创伤或疾病后感觉和运动功能的再生和恢复。