权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

LONG TERM RECORDING FROM AFFERENT TASTE FIBERS

味觉传入纤维的长期记录

基本信息

批准号：
6649331
负责人：
Robert M Bradley
金额：
$ 25.58万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-29 至 2005-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6649331
关键词：
afferent nerve bioengineering /biomedical engineering biomedical equipment development cell cycle chorda tympani electronic recording system implant laboratory rat longitudinal animal study microelectrodes miniature biomedical equipment nervous system regeneration neurofilament single cell analysis taste buds telemetry

项目摘要

Development of an implantable, multielectrode array has made it possible to record chronically, over periods of several weeks, from sensory fibers of taste nerves that innervate the tongue. In the current proposal, the electrode array will be used to systematically test hypotheses about neural discharge properties of peripheral taste fibers over long time frames that encompass numerous cycles of taste bud cell turnover, and synapse remodeling. In addition, the multielectrode recording device will be progressively refined to improve and expand its range of capabilities. The rat chorda tympani nerve will be dissected and implanted with the multielectrode array through which taste nerve fibers regenerate. Subsequently, recordings will be made from the same single fiber, or sets of fibers, for weeks. In Specific Aim 1, chronic recordings from single taste fibers of the chorda tympani nerve will be used to test the hypotheses that response characteristics of taste fibers, and receptive field properties, remain stable during cycles of taste bud cell turnover and synapse remodeling. In Specific Aim 2, simultaneous recordings from several individual fibers will be studied over weeks to test the hypothesis that there is a unique pattern of fiber activity for separate chemical qualities, and that the pattern remains stable over time. In Specific Aim 3, an implantable telemetry system will be used with the multielectrode array to record from chorda tympani fibers in awake rats and investigate the pattern of neural activity during feeding and drinking. The implantable, multielectrode recording array and its further refinement make possible tests of hypotheses about basic taste neurobiology that could not be resolved with previous neurophysiological techniques. Also, studies of taste fiber responses in behaving animals become feasible with the implantable device. Furthermore, continued refinement of, and experiments with, the implantable electrode array can lead to potential applications as a neural prosthesis in regeneration and restoration of sensory and motor function after trauma or disease.

一种可植入的多电极阵列的开发使得在几周的时间内从支配舌头的味觉神经的感觉纤维中进行慢性记录成为可能。在目前的提议中，电极阵列将被用来系统地测试关于外周味觉纤维在长时间框架内的神经放电特性的假说，该假说包括味蕾细胞周转和突触重塑的无数周期。此外，还将逐步改进多电极记录装置，以改进和扩大其能力范围。将大鼠鼓索神经解剖并植入多电极阵列，味觉神经纤维通过多电极阵列再生。随后，将使用相同的单根或多根光纤连续数周进行录制。在具体目标1中，将使用鼓索神经单个味觉纤维的慢性记录来检验味觉纤维的反应特性和感受场特性在味蕾细胞更新和突触重塑周期中保持稳定的假设。在具体目标2中，几个单独纤维的同时记录将在几周内进行研究，以检验以下假设：对于不同的化学特性，纤维活动存在独特的模式，并且该模式随时间保持稳定。在具体目标3中，将使用植入式遥测系统与多电极阵列一起记录清醒大鼠的鼓索纤维，并研究喂养和饮水过程中的神经活动模式。可植入的多电极记录阵列及其进一步的改进使测试有关基本味觉神经生物学的假说成为可能，而这些假说无法用以前的神经生理学技术解决。此外，通过植入设备，研究行为动物的味觉纤维反应也成为可能。此外，对可植入电极阵列的持续改进和实验可能导致作为神经假体的潜在应用，用于创伤或疾病后感觉和运动功能的再生和恢复。