LONG TERM RECORDING FROM AFFERENT TASTE FIBERS

味觉传入纤维的长期记录

• 批准号: 6379509
• 负责人: Robert M Bradley
• 金额: $ 23.79万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-29 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379509
• 关键词: afferent nerve; bioengineering /biomedical engineering; biomedical equipment development; cell cycle; chorda tympani; electronic recording system; implant; laboratory rat; longitudinal animal study; microelectrodes; miniature biomedical equipment; nervous system regeneration; neurofilament; single cell analysis; taste buds; telemetry

Development of an implantable, multielectrode array has made it possible to record chronically, over periods of several weeks, from sensory fibers of taste nerves that innervate the tongue. In the current proposal, the electrode array will be used to systematically test hypotheses about neural discharge properties of peripheral taste fibers over long time frames that encompass numerous cycles of taste bud cell turnover, and synapse remodeling. In addition, the multielectrode recording device will be progressively refined to improve and expand its range of capabilities. The rat chorda tympani nerve will be dissected and implanted with the multielectrode array through which taste nerve fibers regenerate. Subsequently, recordings will be made from the same single fiber, or sets of fibers, for weeks. In Specific Aim 1, chronic recordings from single taste fibers of the chorda tympani nerve will be used to test the hypotheses that response characteristics of taste fibers, and receptive field properties, remain stable during cycles of taste bud cell turnover and synapse remodeling. In Specific Aim 2, simultaneous recordings from several individual fibers will be studied over weeks to test the hypothesis that there is a unique pattern of fiber activity for separate chemical qualities, and that the pattern remains stable over time. In Specific Aim 3, an implantable telemetry system will be used with the multielectrode array to record from chorda tympani fibers in awake rats and investigate the pattern of neural activity during feeding and drinking. The implantable, multielectrode recording array and its further refinement make possible tests of hypotheses about basic taste neurobiology that could not be resolved with previous neurophysiological techniques. Also, studies of taste fiber responses in behaving animals become feasible with the implantable device. Furthermore, continued refinement of, and experiments with, the implantable electrode array can lead to potential applications as a neural prosthesis in regeneration and restoration of sensory and motor function after trauma or disease.

一种可植入的多电极阵列的发展使得人们有可能在几周的时间内长期记录支配舌头的味觉神经的感觉纤维。在目前的提议中，电极阵列将用于系统地测试关于长时间框架内外周味觉纤维的神经放电特性的假设，所述长时间框架包括味蕾细胞周转和突触重塑的许多周期。 此外，多电极记录设备将逐步完善，以提高和扩大其功能范围。 将大鼠鼓索神经解剖并植入多电极阵列，味觉神经纤维通过该阵列再生。 随后，将从相同的单根纤维或多组纤维进行记录，持续数周。 在特定目标1中，将使用来自鼓索神经的单个味觉纤维的慢性记录来测试以下假设：味觉纤维的响应特性和感受野特性在味蕾细胞更新和突触重塑的周期期间保持稳定。 在特定目标2中，将在数周内研究几种单独纤维的同时记录，以检验以下假设：对于不同的化学品质，纤维活性存在独特的模式，并且该模式随时间保持稳定。 在特定目标3中，将使用植入式遥测系统和多电极阵列记录清醒大鼠的鼓索纤维，并研究进食和饮水期间的神经活动模式。 可植入的多电极记录阵列及其进一步的改进使得对以前的神经生理学技术无法解决的基本味觉神经生物学假设的测试成为可能。 此外，在行为动物中研究味觉纤维反应也变得可行。此外，可植入电极阵列的持续改进和实验可以导致作为神经假体在创伤或疾病后的感觉和运动功能的再生和恢复中的潜在应用。