REGULATION OF COLLAGEN MINERALIZATION IN THE TOOTH
REGULATION OF COLLAGEN MINERALIZATION IN THE TOOTH
批准号:
6176164
负责人:
MITSUO YAMAUCHI
金额:
$23.08万
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-07-01 至 2002-03-31
关键词:
SDS polyacrylamide gel electrophoresis animal tissue cementum collagen crosslink dentin dipeptides glycosylation high performance liquid chromatography hydroxylation immunocytochemistry intermolecular interaction normal ossification protein purification protein structure function proteoglycan stereochemistry tendons tooth
中文摘要
描述(改编自研究者摘要):
本研究旨在了解空间调控机制,
和胶原矿化的时间方面。 在上一次赠款期间
阶段,胶原蛋白化学状态的部分表征
交联和胶原纤维的三维结构,
与矿化的关系。 这些发现导致
假设,在基于胶原的矿化组织中,
矿物薄片的堆积在空间上受到
I型非三螺旋结构域(端肽)的构型
胶原分子 此外,一组蛋白聚糖(其中许多是
胶原蛋白相关的蛋白聚糖)已经被鉴定,
几乎只在矿化前被免疫定位
基质,例如近牙周膜细胞、前牙骨质和前牙本质。 这些
蛋白聚糖可能通过其
与胶原纤维结合。 通过使用牙齿(不
改造)作为主要模型,在此提出的具体问题
应用是:氨基和氨基的化学状态是什么?
在矿化前、矿化中的羧基末端非三重螺旋结构域
和非(从不)矿化胶原蛋白? 这些化学状态是如何变化的
与矿化? 的结构特点是什么
蛋白聚糖及其与胶原纤维的空间关系
前牙本质和前牙骨质 它们在成矿基质中是如何变化的
(牙本质和牙骨质)? 为了解决这些问题,生物化学,
物理,免疫化学和免疫组织化学技术将是
就业。 从这些研究中获得的信息将提供洞察力
胶原矿化的调节机制。
英文摘要
DESCRIPTION (Adapted from investigator's Abstract): The long term goal of
this investigation is to understand the mechanisms regulating the spatial
and temporal aspects of collagen mineralization. During the last grant
period, partial characterization of the chemical states of collagen
cross-linking and the three dimensional structure of the collagen fibrils in
relation to mineralization were accomplished. These findings have led to
the hypothesis that, in collagen-based mineralized tissues, the orientation
and stacking of mineral platelets are spatially regulated by the
configuration of the nontriple helical domains (telopeptides) of the type I
collagen molecules. In addition, a group of proteoglycans (many of them are
collagen-associated proteoglycans) in the tooth have been identified and
were found to be immunolocalized almost exclusively in the premineralizing
matrices such as near pericementocytes, precementum and predentin. These
proteoglycans may be inhibiting premature mineralization through their
association with collagen fibrils. By using the tooth (which does not
remodel) as the major model, the specific questions asked in this
application are: What are the chemical states of the amino- and
carboxyl-terminal nontriple helical domains in premineralizing, mineralizing
and non (never)-mineralizing collagens? How do these chemical states change
with mineralization? What are the structural characteristics of the
proteoglycans and their spatial relationship to collagen fibrils in
predentin and precementum? and how do they change in mineralizing matrix
(dentin and cementum)? In order to address these questions, biochemical,
physical, immunochemical and immunohistochemical techniques will be
employed. The information obtained from these studies will provide insight
into the regulatory mechanisms of collagen mineralization.
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会议论文
Collagen Glycosylation, Maturation and Mineralization
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批准号:8249266
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项目类别:
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资助金额:$19.4万
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财政年份:2012
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负责人:MITSUO YAMAUCHI
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依托单位:
Collagen Glycosylation, Maturation and Mineralization
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批准号:8442308
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项目类别:
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资助金额:$15.82万
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财政年份:2012
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负责人:MITSUO YAMAUCHI
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依托单位:
Matrix-assisted Craniofacial Bone Regeneration
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批准号:8197644
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项目类别:
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资助金额:$22.2万
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财政年份:2010
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负责人:MITSUO YAMAUCHI
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依托单位:
Matrix-assisted Craniofacial Bone Regeneration
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批准号:8045684
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项目类别:
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资助金额:$18.4万
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财政年份:2010
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负责人:MITSUO YAMAUCHI
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依托单位:
Lysyl Oxidase Control of TGF-? in Bone
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批准号:7577976
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项目类别:
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资助金额:$22.19万
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财政年份:2009
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负责人:MITSUO YAMAUCHI
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依托单位:
DDR2, a Novel Collagen Receptor, and Mineralization
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批准号:7035336
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项目类别:
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资助金额:$17.82万
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财政年份:2005
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负责人:MITSUO YAMAUCHI
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依托单位:
Biglycan: A novel modulator of osteoblast differentiation
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批准号:7140327
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项目类别:
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资助金额:$15.68万
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财政年份:2005
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负责人:MITSUO YAMAUCHI
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依托单位:
Biglycan: A novel modulator of osteoblast differentiation
-
批准号:6962581
-
项目类别:
-
资助金额:$19.27万
-
财政年份:2005
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MATRIX MINERALIZATION
-
批准号:6817520
-
项目类别:
-
资助金额:$29.2万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MINERALIZATION IN THE TOOTH
-
批准号:6379751
-
项目类别:
-
资助金额:$23.77万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
BIOCHEMISTRY AND MINERALIZATION OF TOOTH CEMENTUM
-
批准号:2131373
-
项目类别:
-
资助金额:$23.15万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MINERALIZATION IN THE TOOTH
-
批准号:2897038
-
项目类别:
-
资助金额:$23.31万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
BIOCHEMISTRY AND MINERALIZATION OF TOOTH CEMENTUM
-
批准号:2131371
-
项目类别:
-
资助金额:$21.59万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MATRIX MINERALIZATION
-
批准号:6720885
-
项目类别:
-
资助金额:$31.98万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MINERALIZATION IN THE TOOTH
-
批准号:2620158
-
项目类别:
-
资助金额:$21.75万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
BIOCHEMISTRY AND MINERALIZATION OF TOOTH CEMENTUM
-
批准号:3223970
-
项目类别:
-
资助金额:$20.58万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
REGULATION OF COLLAGEN MATRIX MINERALIZATION
-
批准号:6984083
-
项目类别:
-
资助金额:$28.51万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
BIOCHEMISTRY AND MINERALIZATION OF TOOTH CEMENTUM
-
批准号:2131372
-
项目类别:
-
资助金额:$22.26万
-
财政年份:1993
-
负责人:MITSUO YAMAUCHI
-
依托单位:
STRUCTURE AND FUNCTION OF DENTIN MATRIX PROTEINS
-
批准号:3072175
-
项目类别:
-
资助金额:$4.93万
-
财政年份:1988
-
负责人:MITSUO YAMAUCHI
-
依托单位:
EXTRACELLULAR MATRIX PROTEINS OF PERIODONTAL LIGAMENT
-
批准号:3222422
-
项目类别:
-
资助金额:$9.13万
-
财政年份:1988
-
负责人:MITSUO YAMAUCHI
-
依托单位:
海外基金