OSTEOBLAST MEDIATED TURNOVER OF COLLAGENASE IN BONE
成骨细胞介导骨中胶原酶的周转
基本信息
- 批准号:6130936
- 负责人:
- 金额:$ 22.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-02-01 至 2004-06-30
- 项目状态:已结题
- 来源:
- 关键词:affinity chromatography collagenase enzyme linked immunosorbent assay gene induction /repression gene mutation laboratory rabbit low density lipoprotein receptor metalloendopeptidases molecular cloning osteoblasts parathyroid hormones physiologic bone resorption plasminogen activator protein sequence protein structure function receptor binding receptor expression site directed mutagenesis
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): The principal
investigator, Nicola Partridge, Ph.D., proposes to examine the mechanism of the
binding, internalization and degradation of the resorptive enzyme,
collagenase-3 (a.k.a., metalloproteinase 13, MMP-13) following its elaboration
from the osteoblast during resorption. It is hypothesized that there is a
specific receptor, the collagenase-3 receptor, which binds to collagenase-3,
resulting in endocytosis that triggers a further interaction of collagenase-3
with the low-density lipoprotein receptor-related protein (LRP). The precise
concentration, and hence, the biological activity of MMP-3 depends on a balance
between its secretion and its receptor-mediated uptake. Based upon preliminary
data that the putative receptor may be a novel lectin, the applicant proposes
four Specific Aims. First, she proposes to express and examine the regulation
of the collagenase-3 receptor. Secondly, she proposes to mutate the receptor to
determine its functional domains that interact with collagenase-3. Thirdly, she
will study the interaction of the collagenase-3 molecule with LRP. Finally, she
proposes to determine the domains of the collagenase-3 molecule that bind the
collagenase-3 receptor and the LRP receptor. Critical new information on the
biology of this important molecule may be derived from these studies.
描述(改编自申请人的摘要): 校长
研究人员 Nicola Partridge 博士提议研究该机制
再吸收酶的结合、内化和降解,
胶原酶 3(又名金属蛋白酶 13、MMP-13)的精制后
在吸收过程中来自成骨细胞。假设有一个
特异性受体,胶原酶 3 受体,与胶原酶 3 结合,
导致内吞作用,引发胶原酶 3 进一步相互作用
与低密度脂蛋白受体相关蛋白(LRP)。精确的
浓度,因此,MMP-3 的生物活性取决于平衡
其分泌和受体介导的摄取之间的关系。根据初步
申请人提出,数据表明推定受体可能是一种新型凝集素
四个具体目标。首先,她建议表达并审查该规定
胶原酶 3 受体。其次,她建议将受体突变为
确定其与胶原酶 3 相互作用的功能域。第三,她
将研究胶原酶 3 分子与 LRP 的相互作用。最后,她
提议确定胶原酶 3 分子结合的结构域
胶原酶3受体和LRP受体。有关的重要新信息
这种重要分子的生物学可能源自这些研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Nicola C Partridge其他文献
Nicola C Partridge的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Nicola C Partridge', 18)}}的其他基金
Skyscan 1172 Ex-vivo MicroComputed Tomography System
Skyscan 1172 离体微型计算机断层扫描系统
- 批准号:
8446705 - 财政年份:2013
- 资助金额:
$ 22.84万 - 项目类别:
相似海外基金
Exploring the effects of actinidin as a novel collagenase on Human peyronie's disease cells
探索猕猴桃素作为一种新型胶原酶对人类佩罗尼氏病细胞的影响
- 批准号:
574530-2022 - 财政年份:2022
- 资助金额:
$ 22.84万 - 项目类别:
University Undergraduate Student Research Awards
Investigating the effect of collagenase on the passive and active properties of skeletal muscle in children with cerebral palsy
探讨胶原酶对脑瘫儿童骨骼肌被动和主动特性的影响
- 批准号:
486139 - 财政年份:2022
- 资助金额:
$ 22.84万 - 项目类别:
Studentship Programs
Elucidating the role and mechanisms by which collagenase producing intestinal bacteria promote colorectal cancer recurrence and metastasis following surgery.
阐明产胶原酶肠道细菌促进结直肠癌术后复发和转移的作用和机制。
- 批准号:
10547761 - 财政年份:2021
- 资助金额:
$ 22.84万 - 项目类别:
Elucidating the role and mechanisms by which collagenase producing intestinal bacteria promote colorectal cancer recurrence and metastasis following surgery.
阐明产胶原酶肠道细菌促进结直肠癌术后复发和转移的作用和机制。
- 批准号:
10320461 - 财政年份:2021
- 资助金额:
$ 22.84万 - 项目类别:
Investigating the impact of collagenase in Clostridioides difficile pathogenesis
研究胶原酶在艰难梭菌发病机制中的影响
- 批准号:
10583447 - 财政年份:2020
- 资助金额:
$ 22.84万 - 项目类别:
Surface modification of the cubic micro-cartilage by collagenase treatment and its efficacy in cartilage regeneration for ear tissue engineering
胶原酶处理立方体微软骨的表面修饰及其在耳组织工程软骨再生中的功效
- 批准号:
20K18425 - 财政年份:2020
- 资助金额:
$ 22.84万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Widespread injury of intestinal serosa with type I collagenase promote colonization of transplanted enteric neural stem cells
I型胶原酶对肠浆膜的广泛损伤促进移植肠神经干细胞的定植
- 批准号:
17K17008 - 财政年份:2017
- 资助金额:
$ 22.84万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Catheter Based Ultrasound Mediated Collagenase Therapy for the Treatment of Chronic Total Occlusions
基于导管的超声介导的胶原酶疗法治疗慢性完全闭塞
- 批准号:
351824 - 财政年份:2016
- 资助金额:
$ 22.84万 - 项目类别:
Operating Grants
Acceleration of fracture healing by growth factor fused with tandem collagen-binding domains from Clostridium histolyticum collagenase
生长因子与溶组织梭菌胶原酶串联胶原结合域融合加速骨折愈合
- 批准号:
15K20015 - 财政年份:2015
- 资助金额:
$ 22.84万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Effects of the Collagenase of the Bacteria Clostridium Histolyticum on the Development of Capsular Fibrosis after Insertion of Silicone Implants in Rats
溶组织梭菌胶原酶对大鼠硅胶植入物植入后包膜纤维化发展的影响
- 批准号:
259236300 - 财政年份:2015
- 资助金额:
$ 22.84万 - 项目类别:
Research Fellowships