NOREPINEPHRINE AND ATTENTIONAL MODULATION OF CORTEX

去甲肾上腺素和皮质注意力调节

• 批准号: 6137823
• 负责人: RALPH M SIEGEL
• 金额: $ 7.8万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6137823
• 关键词: Primates; attention; behavior test; behavioral /social science research tag; electrophysiology; hormone regulation /control mechanism; locus coeruleus; microdialysis; neurochemistry; neuropharmacology; norepinephrine; parietal lobe /cortex; secretion; visual stimulus

DESCRIPTION (Applicant's abstract): The parietal cortex is a visual region that represents spatial relationships. This representation has been shown dependent on attentional state in both human and non-human primates. In the latter species, electrophysiological measurements have repeatedly shown that "attention" can alter the response of single cells in the inferior parietal lobule. Few studies have examined what physiological mechanisms underlie the modulation of the visual response. The lacuna in understanding is not limited to the parietal cortex as similar issues exist for other cortices. The current proposal will be the first to use precise neuropharmacological methods to address the issue of attentional modulation of visual responses in the cortex of behaving monkeys. The approach will be three-fold. Behavioral, electrophysiological and neuropharmacological methods will be combined in order to examine how the locus coeruleus diffuse projections may alter cortical responses. The primary hypothesis is that norepinephrine release underlies attentional modulation of neural activity in parietal cortex. Specifically norepinephrine release varies with locus coeruleus activity altering signal-to-noise processing capabilities of individual pyramidal neurons and the functional circuitry of interneurons. To test this hypothesis, monkeys will be trained to perform a behavioral task in which the attentional dimensions of difficulty and focus may be systematically manipulated. The strength and the spatial distribution of the neural representation will be quantitatively evaluated as a function of attentional state using single unit recording methods. Microdialysis will then be used to measure extracellular norepinephrine levels. Pharmacological manipulations will be performed to confirm that norepinephrine is being released at this site. Then, norepinephrine release at different attentional states will be measured. Correlations will be drawn between the electrophysiological and pharmacological measures. Strategies for restoring attentional deficits may be derived from this work.

描述（申请人的摘要）：顶叶是一个视觉 代表空间关系的区域。这个表示有 显示了人类和非人类的注意力状态 灵长类动物。在后一种物种中，电生理测量具有 反复表明“注意”可以改变单个单元的响应 在下顶叶。很少有研究检查了什么 生理机制是视觉反应调节的基础。 理解的空隙不仅限于顶叶皮质 其他皮质也存在类似的问题。当前的建议将是 第一个使用精确的神经药物方法来解决 视觉反应的注意力调制的问题 行为猴子。该方法将是三倍。行为， 电生理和神经药物方法将合并 为了检查基因座二元扩散投影如何 改变皮质反应。主要假设是去甲肾上腺素 释放是对顶叶神经活动的注意调制的基础 皮质。特异性去甲肾上腺素的释放随层层植物而变化 各个个人的信号处理功能改变了个人的活动 锥体神经元和中间神经元的功能电路。测试 这个假设，猴子将接受训练以执行行为任务 其中的注意力和重点可能是 系统地操纵。强度和空间分布 神经代表的形式将被定量评估为 使用单个单元记录方法的注意状态的功能。 然后，微透析将用于测量细胞外去甲肾上腺素 水平。将进行药理操作，以确认 去甲肾上腺素正在此站点发布。然后，去甲肾上腺素 将测量在不同注意状态下的释放。相关性 将在电生理和药理之间绘制 措施。可以得出恢复注意力缺陷的策略 从这项工作。