NEURAL CIRCUITS OF THE INFERIOR PARIETAL LOBULE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该提案的目标是直接检验米奇森和克里克斯关于行为猴顶下小叶功能回路组织的假设。为了研究体内顶下小叶的功能回路组织，我们在过去三年里完善了对行为猴的基因改造神经元进行双光子成像的能力。这些神经元被病毒转染以表达电压或钙的荧光传感器。因此，可以以微观分辨率对神经元及其神经元活动进行成像。进行这些实验已经证明了行为猴制备的许多优点。我们观察了距软脑膜表面已知深度的神经元，观察了它们在切向平面上彼此之间的精确空间关系，并测量了它们在行为任务期间的调谐。事实上，我们已经对 15 个神经元进行了 3 小时的稳定连续双光子显微记录。这些进展支持在行为过程中检查关联皮层中的特定投射功能回路。我们的工作假设是：顶下小叶被组织成局部模块，其中包含具有相同投影模式和相同功能的神经元。我们将利用这些资金对生物实验中收集的大型成像数据集进行计算分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this proposal is to directly examine Mitchison and Cricks hypothesis in terms of the functional circuit organization of the inferior parietal lobule in behaving monkey. To study the functional circuit organization of inferior parietal lobule in vivo, we have spent the last three years perfecting the ability to perform two-photon imaging of genetically transformed neurons in the behaving monkey. These neurons are virally transfected to express a fluorescent sensor for voltage or calcium. Thus a neuron can be imaged at microscopic resolution along with its neuronal activity. Performing these experiments has demonstrated many advantages for the behaving monkey preparation. We have observed neurons a known depth from the pial surface, observed their precise spatial relationships to each other in the tangential plane, and measured their tuning during a behavioral task. Indeed, we have made stable continuous two-photon microscopic recordings from 15 neurons for 3 hours. These advances support examining specific projective functional circuits in association cortex during behavior. Our working hypothesis is: the inferior parietal lobule is organized into local modules containing neurons with the same projection patterns and the same functions. We will be using these funds to computationally analyze the large imaging data sets collected in the biological experiments.