THE S. CEREVISIAE S PHASE CHECKPOINT

描述：(改编自《调查者摘要》)。这项提案将使用 探索协调DNA完成的检查点的酵母模型系统 有丝分裂的复制。具体地说，这项提案将调查如何 酵母细胞感觉到复制尚未完成，就阻止了过渡 从中期到后期。这个检查站的重要性显而易见，在 在复制之前进入细胞分裂过程 遗传物质是一个灾难性的事件，导致瞬间失去 基因组的完整性。该提案集中在几种保守的蛋白质上，这些蛋白质 通过基因分析鉴定，其中一些以前没有 与检查点功能关联。具体的目标是描述 依赖Pds1延时的“迟”S相位检查站的参数 晚期，探讨RAD23和Ddi1在PDS1依赖的S中的作用 阶段检查站并进行详细的结构/功能分析 RAD23和Ddi1蛋白。

DESCRIPTION: (Adapted from the Investigator's Abstract). This proposal will use a yeast model system to explore a checkpoint that coordinates completion of DNA replication with mitosis. Specifically, this proposal will investigate how a yeast cell, sensing that replication is not yet complete, blocks the transition from metaphase to anaphase. The importance of this checkpoint is obvious, in that entry into the process of cell division prior to duplication of the genetic material is a catastrophic event, leading to instantaneous loss of genomic integrity. The proposal focuses on several conserved proteins that were identified through genetic analysis, some of which previously had not been associated with checkpoint functions. The specific aims are to characterize the parameters of a "late" S phase checkpoint that depends on Pds1 to delay anaphase, to investigate the role of Rad23 and Ddi1 in the Pds1-dependent S phase checkpoint and to engage in a detailed structure/function analysis of the Rad23 and Ddi1 proteins.