MOLECULAR DYNAMICS AT THE DENTIN/ADHESIVE INTERFACE

牙本质粘结的目的有两个，即提取矿物相 而不会破坏胶原蛋白，也不会渗入 矿物与粘合树脂。如果这个理想实现了，胶原蛋白 脱矿的牙本质纤维会被树脂包裹，因此， 防止口腔或牙本质液的水解性作用。那里 是否有确凿证据表明这一目标并未实现 研究人员在应该使用什么技术来实现这一点上存在分歧 理想的牙本质/粘结剂粘结剂。 到目前为止，牙本质/粘附剂粘结的研究主要是使用 结合结合强度测量进行形态分析。 虽然这些分析加深了我们的理解，但许多 有关牙本质/粘接剂界面的化学问题如下 无人接听。拉曼光谱是一种光散射技术， 中存在的分子物种提供“指纹”。 样本。当这项技术与显微镜相结合时，化合物- 特定分子可以在横向空间分辨率为 大约1微米。尽管背景荧光传统上 限制了拉曼光谱的使用，这个实验室所做的工作 最近的研究表明，荧光的干扰可以通过以下方法避免 使用红色激励源。利用微拉曼光谱和Kr+ 激光绘制了牙本质/混合体/粘结剂界面的化学图谱 建造的。这个项目的总体假设是，一个前- 治疗方案，提供完整的树脂包裹体 脱矿层中的胶原蛋白将产生持久的牙本质/ 粘性粘合剂。可以确定最佳的前处理方案 使用互补的化学、形态和机械数据。这个 这项研究的具体目的是：检验牙本质 用自酸蚀底漆制备，可防止胶原蛋白崩解和 这种粘接树脂可以穿透以这种方式处理的牙本质；测试 胶粘树脂完全包裹胶原蛋白的假说 自酸蚀底物处理牙本质后暴露出的纤维； 验证自酸蚀底漆可提供优质树脂的假设 裸露胶原蛋白的穿透和包封性比较 用稳定剂，特别是丙酮处理酸蚀牙本质 和HEMA；检验自酸蚀底漆提供更强的 牙本质/粘结剂粘结与酸蚀牙本质粘结的比较 稳定溶剂；检验与蚀刻相比的假设 用稳定剂处理的牙本质自酸蚀底漆提供 牙本质/粘附剂粘结在水溶液中稳定 环境。

The goal of dentin bonding is twofold, i.e. to extract the mineral phase without damaging the collagen and impregnate the voids left by the mineral with adhesive resin. If this ideal were achieved, the collagen fibers of demineralized dentin would be enclosed in resin and thus, protected from the hydrolytic action of oral or dentinal fluids. There is substantial evidence to indicate that this objective is not achieved and researchers disagree on what techniques should be used to accomplish the ideal dentin/adhesive bond. To date, the dentin/adhesive bond has primarily been studied using morphologic analysis in conjunction with bond strength measurement. Although these analyses have enhanced our understanding, numerous questions about the chemistry of the dentin/adhesive interface are unanswered. Raman spectroscopy is a light scattering technique that provides a "fingerprint" for the molecular species present within a sample. When this technique is combined with a microscope, compound- specific molecules can be detected at a lateral spatial resolution of approximately 1mum. Although background fluorescence has traditionally limited the use of Raman spectroscopy, work done in this laboratory has recently shown that interference from fluorescence can be avoided by using a red excitation source. Using micro-Raman spectroscopy and a Kr+ laser a chemical map of the dentin/hybrid/adhesive interface has been constructed. The overall hypothesis of this project is that a pre- treatment regimen which provides complete resin encapsulation of the collagen in the demineralized zone will result in durable dentin/ adhesive bonds. The optimum pre-treatment regimen can be determined using complementary chemical, morphologic, and mechanical data. The specific aims of this study are: test the hypothesis that dentin prepared with self etching primers will resist collagen collapse and that adhesive resin can penetrate dentin treated in this manner; test the hypothesis that adhesive resin completely encapsulates collagen fibrils exposed after treatment of the dentin with self etching primers; test the hypothesis that self-etching primers provide superior resin penetration and encapsulation of exposed collagen in comparison to etched dentin treated with stabilizing solvents, specifically acetone and HEMA; test the hypothesis that self-etching primers provide stronger dentin/adhesive bonds in comparison to etched dentin treated with stabilizing solvents; test the hypothesis that in comparison to etched dentin treated with stabilizing solvents self-etching primers provide dentin/adhesive bonds that are stable upon exposure to aqueous environments.