MECHANISMS OF D/V PATTERNING DURING NEUROGENESIS

神经发生过程中 D/V 模式的机制

• 批准号: 6012449
• 负责人: TONIA L VON OHLEN
• 金额: $ 3.75万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6012449
• 关键词: DNA binding protein; Drosophilidae; biochemical evolution; developmental genetics; developmental neurobiology; dorsal column; gene expression; genetic mapping; genetic regulation; neurogenesis; neurogenetics; phenotype; ventral column; vertebrate embryology; zebrafish

In both vertebrates and Drosophila suggest that similar positional cues regulate dorsal ventral patterning within the CNS. During formation of the Drosophila CNS NB's form in three rows parallel to the ventral midline. Initially the ventral and dorsal columns form, followed by filling in of the intermediate column. ventral nervous system defective (vnd) is expressed in the ventral NB column, and muscle segment homeobox (msh) is expressed in the lateral column. While vnd plays a key role in formation of the medial column NBs and msh plays a similar role in the lateral column it remains unclear if there is a similar factor regulating formation of the intermediate column NBs. This project focuses on defining a role for a newly discovered gene, intermediate neuroblasts defective (ind). indis a novel homeodomain protein expressed in the intermediate column. Characterization of ind will include determination of the loss and gain of function phenotypes and identification of downstream targets. In parallel I will test two putative murine homologs (Gsh 1 and Gsh 2) for functional conservation.

在脊椎动物和果蝇表明，类似的位置线索调节中枢神经系统内的背腹图案。 在果蝇中枢神经系统的形成过程中，NB的形式是平行于腹侧中线的三排。 最初腹柱和背柱形成，随后填充中间柱。 腹神经系统缺陷（vnd）在腹侧NB柱中表达，而肌肉节段同源框（msh）在侧柱中表达。 虽然vnd在内侧柱NB的形成中起关键作用，并且msh在外侧柱中起类似作用，但仍不清楚是否存在调节中间柱NB的形成的类似因素。 该项目的重点是确定一个新发现的基因，中间神经母细胞缺陷（ind）的作用。indis是一种新的同源结构域蛋白表达的中间柱。ind的表征将包括确定功能表型的丧失和获得以及下游靶点的鉴定。 同时，我将测试两个假定的小鼠同源物（GSH 1和GSH 2）的功能保守性。