IMMUNOLOGICAL MECHANISM OF INFLAMMATION IN UVEITIS & EAE

描述（改编自申请人的摘要）：葡萄膜炎是一组 可能由感染，恶性肿瘤， 创伤，或不完全理解的过程，可能是由 身体的免疫系统。 在许多情况下，葡萄膜炎与 潜在的炎症性疾病过程在身体的其他地方。 葡萄膜炎 也可能与多发性硬化症（MS）有关， 神经系统（CNS）炎症与神经系统炎症有关。 眼睛 在这些研究中，建议澄清 炎症在眼睛和中枢神经系统，这可能会给线索， 这种炎症性疾病的发展机制。 它还可以提供 一个有用的模型，用于研究眼睛炎症作为人类的并发症， 全身性疾病。 为此，建议使用动物模型 对于MS-EAE。 与一些MS患者类似，动物会发展为 眼睛的炎症与眼睛的炎症有关。 脊髓 在刘易斯大鼠中，第二次给药可再次诱导葡萄膜炎 髓鞘碱性蛋白（MBP） 因此，该动物模型允许 研究复发性葡萄膜炎的机制，这在人类中导致 永久性组织损伤和失明 建议测试 假设有共同的致病性自身免疫机制参与 在眼睛和CNS的炎症中，包括常见的靶抗原。 因此，在所提出的模型中，MBP特异性T细胞在免疫应答中发挥作用。 引发导致葡萄膜炎的事件以及 眼睛发炎 申请人假设，不仅细胞， 吸引这些细胞到眼睛的因素也是 致病过程 为了阐明涉及的免疫学机制， 在葡萄膜炎的发病机理中，他们提出了以下具体目标： (1)确定免疫应答对靶抗原的特异性， 免疫后同时发生前葡萄膜炎和EAE的刘易斯大鼠 关于MBP (2)明确T细胞在刘易斯复发性葡萄膜炎中的作用 用MBP重新检查大鼠。 (3)检查细胞因子的作用， 趋化因子在原发性和复发性眼内细胞募集中的作用 MBP免疫刘易斯大鼠后EAE相关的葡萄膜炎。

DESCRIPTION (Adapted from applicant's abstract): Uveitis is a group of inflammatory eye diseases that may result from infections, malignancies, trauma, or incompletely understood processes that are presumably mediated by the body's immune system. In many cases, uveitis is associated with underlying inflammatory disease processes elsewhere in the body. Uveitis may be also associated with multiple sclerosis (MS) where the central nervous system (CNS) inflammation is associated with the inflammation of the eye. In these studies, it is proposed to clarify the relationship between inflammation in the eye and in the CNS, which may give a clue about the mechanism of development of this inflammatory disease. It may also provide a useful model for studying an eye inflammation as a complication of human systemic disorders. For this reason, it is proposed to use an animal model for MS-EAE. Similarly to some patients with MS, animals develop inflammation of the eye that is associated with the inflammation of the spinal cord. In Lewis rats, uveitis can be re-induced with the second dose of myelin basic protein (MBP). Therefore, this animal model permits the study of the mechanism of relapsing uveitis, which in humans leads to permanent tissue damage and blindness. It is proposed to test the hypothesis that there are common pathogenic autoimmune mechanisms involved in inflammation of the eye and the CNS, including common target antigens. Thus, in the proposed model, MBP-specific T cells play a role in the initiation of the events leading to uveitis as well as in the recurrence of eye inflammation. The applicants hypothesize that not only the cells but also factors that attract those cells to the eye are important components of the pathogenic process. To elucidate the immunological mechanism involved in the pathogenesis of uveitis, they propose the following specific aims: (1) Determine specificity of the immune response for a target antigen in Lewis rats which develop both anterior uveitis and EAE after immunization with MBP. (2) Define the role of T cells in the recurrent uveitis in Lewis rats upon re-examination with MBP. (3) Examine the role of cytokines and chemokines in the recruitment of cell to the eye in primary and recurrent uveitis associated with EAE after immunization of Lewis rats with MBP.