NOVEL CLINICAL CHEMISTRY SENSING MOTIF

新颖的临床化学传感基序

• 批准号: 6180893
• 负责人: SANFORD A. ASHER
• 金额: $ 19.43万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180893
• 关键词: acrylamides; biomaterial development /preparation; clinical chemistry; clinical research; fiber optics; gel; human tissue; ions; measurement; polymers

We will develop novel, simple, inexpensive clinical chemistry sensing materials which can be used as visual color test strips to sensitively and selectively report on the concentration and identity of important analytes such as Pb2+, K+, Hg2+, pH, Ca2+, etc. We will develop inexpensive clinical test strips which can be immersed in bodily fluids to accurately determine analytes at the patient bedside. We will also develop arrays of these chemical sensing materials which will be attached to fiber optic bundles to be used as multichannel optrodes to simultaneously monitor numerous analytes. These sensing materials are based on the intelligent polymerized crystalline colloidal array technology we recently developed. This innovative sensing motif utilizes a mesoscopically periodic array of colloidal particles polymerized into an acrylamide hydrogel. This array diffracts light in the visible spectral region due to the periodic array of colloidal particles. This material also contains chelating agents tailored for the analytes of interest. When an analyte binds, its charge is immobilized within the acrylamide hydrogel. The resulting Donnan potential causes an osmotic pressure, which swells the hydrogel array in proportion to the concentration of analyte bound. This results in shifts in the diffracted wavelength and in the perceived color changes. The change in the wavelength diffracted reports on the identity and concentration of the target analyte. This motif is highly flexible and can be modified to sense a broad variety and concentration of biologically relevant analytes.

我们将开发新的、简单、廉价的临床化学传感材料，可用作视觉颜色测试条，以灵敏和选择性地报告重要分析物如Pb2+、K+、Hg2+、pH、Ca~(2+)等的浓度和特性。我们将开发可浸泡在体液中的廉价临床测试条，以准确地测定患者床边的分析物。我们还将开发这些化学传感材料的阵列，这些材料将连接到光纤束上，用作多通道光学电极，以同时监测大量分析物。这些传感材料是基于我们最近开发的智能聚合晶体胶体阵列技术。这一创新的传感基元利用了聚合成丙烯酰胺水凝胶的介观周期性胶体颗粒阵列。由于胶体颗粒的周期性阵列，该阵列在可见光谱区衍射光。该材料还包含为感兴趣的分析物量身定做的螯合剂。当分析物结合时，它的电荷被固定在丙烯酰胺水凝胶中。由此产生的Donnan电势导致渗透压，渗透压与结合分析物的浓度成比例地膨胀水凝胶阵列。这导致了衍射波长的移动和可感知的颜色变化。波长的变化反映了目标分析物的特性和浓度。该基序具有很高的灵活性，可以被修改以检测广泛的生物相关分析物的种类和浓度。