Novel Clinical Chemistry Sensing Motif

新型临床化学传感基序

• 批准号: 7036877
• 负责人: SANFORD A. ASHER
• 金额: $ 32.66万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7036877
• 关键词: ammonia; blood; clinical research; urea cycle

DESCRIPTION (provided by applicant): The development and utilization of home testing has revolutionized the management of chronic diseases such as diabetes mellitus. We propose here a plan to develop a home test kit for analytes such as ammonia and amino acids that would revolutionize the management of aminoacidopathies, urea cycle disorders, and other inborn errors of metabolism. These diseases (for example, PKU) require complex testing for diagnosis and clinical monitoring. While uncommon, individuals suffering from these disorders consume significant amounts of expensive medical care. Treatment requires frequent monitoring of serum amino acid levels, and in the case of the urea cycle disorders, ammonia, to avoid significant morbidity and mortality. In fact the recent NIH consensus statement for PKU stated, "Development of a reliable home-testing method is recommended, as well as measures to increase adherence." In order to improve the care of patients with these inborn errors of metabolism, we will develop photonic crystal sensors for ammonia and specific amino acids that patients could use for home monitoring. Our objective is to develop a sensing materials that can be incorporated into a device that utilizes a small amount of blood from a fingerstick. The blood would be placed on a photonic crystal film sensor that would rapidly change color in response to the concentration of a particular amino acid and/or NH4*. The analyte sensing will be monitored by using a simple Palm Pilot-type instrument that utilizes a color CCD camera. The results could be immediately displayed, and easily recorded if the device is operated as a patient biographer.

描述（由申请人提供）：家庭检测的开发和利用彻底改变了糖尿病等慢性病的管理。我们在这里提出了一个计划，开发一个家庭测试试剂盒的分析物，如氨和氨基酸，将彻底改变管理的氨基酸，尿素循环障碍，和其他先天性代谢缺陷。这些疾病（例如PKU）需要复杂的诊断和临床监测测试。虽然不常见，但患有这些疾病的个体消耗大量昂贵的医疗护理。治疗需要经常监测血清氨基酸水平，在尿素循环障碍的情况下，监测氨，以避免显著的发病率和死亡率。事实上，最近NIH对北大的共识声明指出，“建议开发一种可靠的家庭测试方法，以及增加依从性的措施。“为了改善这些先天性代谢缺陷患者的护理，我们将开发用于氨和特定氨基酸的光子晶体传感器，患者可以用于家庭监测。我们的目标是开发一种传感材料，可以结合到一个设备，利用少量的血液从手指针刺。血液将被放置在光子晶体膜传感器上，该传感器将响应于特定氨基酸和/或NH 4 * 的浓度而快速改变颜色。分析物传感将通过使用简单的Palm Pilot型仪器进行监测，该仪器采用彩色CCD摄像机。结果可以立即显示，并且如果该设备作为患者传记作者操作，则很容易记录。