DISCRIMINATION OF COMPLEX MIXTURE IN 0LFACTORY SIGNALING

0LFACTORY 信令中复杂混合物的判别

• 批准号: 6262879
• 负责人: CHARLES E LINN
• 金额: $ 10万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6262879
• 关键词: Lepidoptera; afferent nerve; cell type; limbic system; neurons; olfactions; pheromone; sensory discrimination; vomeronasal systems

Olfactory glomeruli are the common functional elements in vertebrate and invertebrate neural pathways involved in integrating sensory information about complex odor mixtures. Previous studies have shown that insects can be excellent models for studying the functional role of glomeruli. One of the most studied involves the sex pheromone-processing pathways in the male-specific macroglomerular complex (MGC) of the antennal lobe of male moths. Olfactory glomeruli in the MGC are critically involved in the spatial organization of sensory axons for specific pheromone components and their interactions with specific local and projection interneurons that integrate key aspects of odor quality, quantity, and intermittency. The objectives of the present proposal are to test hypotheses about the interactions of sensory afferents and central neuron types in the olfactory glomeruli, utilizing the technique of transplantation of antennal imaginal disks between males and females of pheromone races of the European corn borer moth (ECB), Ostrinia nubilalis, and two heliothine moths, the tobacco budworm, Heliothis virescens, and the corn earworm, Helicoverpa zea. These two insect systems have been the subject of extensive studies on the genetics of pheromone production and perception (ECB), and electrophysiological/anatomical studies of neural pathways involved in processing the pheromone signal (the heliothines). The antennal transplant procedure will allow us to construct males and females with unique antennal and behavioral response phenotypes to test a model for glomerular organization and behavioral discrimination of odor quality. These interspecies antennal transplants represent a new experimental paradigm for studying basic aspects of the structural and functional role of glomeruli in olfactory discrimination.

嗅球是脊椎动物和无脊椎动物神经通路中的共同功能元件，参与整合复杂气味混合物的感觉信息。以往的研究表明，昆虫可以成为研究肾小球功能作用的极好模型。 其中研究最多的是雄蛾触角叶的雄性特异性巨球复合体（MGC）中的性信息素加工途径。 MGC中的嗅球关键地参与了特定信息素组分的感觉轴突的空间组织以及它们与特定的局部和投射中间神经元的相互作用，所述中间神经元整合了气味质量、数量和不稳定性的关键方面。 本建议的目的是测试假设的相互作用的感觉传入和中枢神经元类型的嗅球，利用技术的触角成虫盘之间的男性和女性的信息素种族的欧洲玉米螟蛾（ECB），玉米螟，和两个太阳神蛾，烟夜蛾，Heliothis virescens，和玉米穗虫，Helicoverpa zea。 这两种昆虫系统已经成为广泛研究信息素产生和感知（ECB）的遗传学，以及参与处理信息素信号（heliothines）的神经通路的电生理学/解剖学研究的主题。 触角移植程序将使我们能够构建具有独特的触角和行为反应表型的雄性和雌性，以测试肾小球组织和气味质量行为辨别的模型。 这些种间触角移植代表了一个新的实验范式，研究基本方面的结构和功能的肾小球在嗅觉歧视的作用。

项目成果

Unusual pheromone receptor neuron responses in heliothine moth antennae derived from inter-species imaginal disc transplantation.

来自种间成虫盘移植的夜蛾触角中异常的信息素受体神经元反应。

• DOI: 10.1007/s00359-002-0371-1
• 发表时间: 2003
• 期刊: Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology.
• 作者: Ochieng,SA;Poole,K;LinnJr,CE;Vickers,NJ;Roelofs,WL;Baker,TC
• 通讯作者: Baker,TC

Consequences of interspecies antennal imaginal disc transplantation on organization of olfactory glomeruli and pheromone blend discrimination.

种间触角成虫盘移植对嗅觉肾小球组织和信息素混合辨别的影响。

• DOI: 10.1002/cne.10890
• 发表时间: 2003
• 期刊: The Journal of comparative neurology.
• 作者: Vickers,NeilJ;Poole,Kathy;LinnJr,CharlesE
• 通讯作者: LinnJr,CharlesE

Plasticity in central olfactory processing and pheromone blend discrimination following interspecies antennal imaginal disc transplantation.

种间触角成虫盘移植后中枢嗅觉处理和信息素混合辨别的可塑性。

• DOI: 10.1002/cne.20725
• 发表时间: 2005
• 期刊: The Journal of comparative neurology
• 作者: Vickers,NeilJ;Poole,Kathy;LinnJr,CharlesE
• 通讯作者: LinnJr,CharlesE