NOVEL APPROACHES TO IMPROVE AND EXTEND PLATELET STORAGE

提出了新的方法来提高储存血小板的质量 并延长血小板储存的持续时间。 将评价广泛的储存条件。 两个主要的假设将被测试，可以解释渐进的 储存过程中血小板活力的丧失：1） 血小板在储存期间不符合;和/或2）血小板 在收集、处理和储存过程中逐渐激活 导致输血后血小板迅速清除。 计划进行研究，以测试输血后的活力和功能 血小板储存在旨在满足代谢需要的溶液中， 加入或不加入活化抑制剂。 将使用已被证实的试剂评价血小板的长期储存。 记录以保持各种细胞和组织的完整性 在低温保存和/或冷冻干燥（冻干）期间。 其他 这些方法包括将血小板加热到37 ℃一小时， 输血以促进任何可逆性储存诱导的 以前导致立即切除受损的 血小板从循环中排出。 我们还将收集和 储存来自先前已给予 血小板生成素（MGDF），以增加他们的血小板计数。 是 假设从这些MGDF刺激的供体中采集的血小板 将包含一群年轻的血小板， 在液体储存期间和/或 低温保存或冷冻干燥。 几种新的仪器和测定方法，可以更好地预测后- 输注血小板的活性和功能比目前可用的 将测试体外测定与后 输血血小板回收率、存活率和出血时间测量。

Novel approaches are proposed to improve the quality of stored platelets at any storage interval and to extend the duration of platelet storage. A broad spectrum of storage conditions will be evaluated. Two major hypotheses will be tested that may explain the progressive loss of platelet viability during storage: 1) the metabolic needs of platelets are not being met during storage; and/or 2) platelets are progressively activated during the collection, processing, and storage of platelets leading to rapid platelet removal following transfusion. Studies are planned to test the post-transfusion viability and function of platelets stored in solutions designed to meet the metabolic needs of platelets with or without the addition of activation inhibitors. Long-term platelet storage will be evaluated using agents that have been documented to maintain the integrity of a variety of cells and tissues during cryopreservation and/or freeze-drying (lyophilization). Other approaches involve warming platelets to 37 C for one hour prior to transfusion to facilitate repair of any reversible storage-induced lesions that previously resulted in the immediate removal of a damaged population of platelets from circulation. We will also collect and store platelets from apheresis donors who have been previously given thrombopoietin (MGDF) to increase their platelet counts. It is hypothesized that platelets harvested from these MGDF-stimulated donors will contain a population of younger platelets that may be more resistant to injury during both liquid storage, and/or during cryopreservation or freeze-drying. Several new instruments and assays that may better predict post- transfusion platelet viability and function than the currently-available in vitro assays will be tested for their correlation with post- transfusion platelet recovery, survival, and bleeding time measurements.