ELECTRICAL SEQUENCING OF DNA

描述（改编自研究者摘要）：研究者的目的 是开发一种微型库尔特计数器， ssDNA被提取。当不同的碱基通过 通道，通过DNA泄漏的电流和停留时间将是 不同的基地。他们建议使用高变性的ssDNA， 温度和非水溶剂。流体流动和/或电梯度 在阅读之前，移液器将拉直DNA。各种方案已经 拟议解决联机记录方面的预期问题 条件，DNA比对条件，移液管表面的化学修饰 和/或聚合物，以及使用胶乳珠粒以实现所需的 单碱基分辨率和四个DNA碱基之间的信号差异。的 该方法可以扩展到RNA和蛋白质测序。

DESCRIPTION (Adapted from the Investigator's Abstract): The investigator's aim is to develop a miniaturized Coulter counter through which a single chain of ssDNA is drawn. As different bases pass through the narrow portion of the channel, the current leaking through the DNA and the residence time will be different for the different bases. They propose to use ssDNAs denatured by high temperatures and non-aqueous solvents. Fluid flow and/or electrical gradient in the pipette will straighten the DNA prior to reading. Various schemes have been proposed to resolve anticipated problems in terms of on-line recording conditions, DNA alignment conditions, chemical modification of pipette surface and/or the polymer, and the use of latex beads in order achieve the required one-base resolution and the signal differences among the four DNA bases. The method could be extended to RNA and protein sequencing.