ELECTRICAL SEQUENCING OF DNA

描述(改编自《调查员摘要》)：调查员的目的 是开发一种小型化的库尔特计数器，通过它可以 单链DNA被绘制出来。当不同的碱基穿过狭窄的 通道，通过DNA泄漏的电流和停留时间将是 对于不同的基地来说是不同的。他们建议使用HIGH变性的单链DNA 温度和非水溶剂。流体流动和/或电势梯度 移液管将在读取前拉直DNA。各种不同的计划已经 建议在在线记录方面解决预期的问题 条件、DNA比对条件、移液管表面化学修饰 和/或聚合物，并使用乳胶珠以达到所要求的 一碱基分辨率和四个DNA碱基之间的信号差异。这个 该方法可推广到RNA和蛋白质测序。

DESCRIPTION (Adapted from the Investigator's Abstract): The investigator's aim is to develop a miniaturized Coulter counter through which a single chain of ssDNA is drawn. As different bases pass through the narrow portion of the channel, the current leaking through the DNA and the residence time will be different for the different bases. They propose to use ssDNAs denatured by high temperatures and non-aqueous solvents. Fluid flow and/or electrical gradient in the pipette will straighten the DNA prior to reading. Various schemes have been proposed to resolve anticipated problems in terms of on-line recording conditions, DNA alignment conditions, chemical modification of pipette surface and/or the polymer, and the use of latex beads in order achieve the required one-base resolution and the signal differences among the four DNA bases. The method could be extended to RNA and protein sequencing.