CORE--NON-INVASIVE MEASUREMENT OF BONE MINERAL

骨矿物质测量核心是骨量测所必需的。 在人类和动物中都有质量。核心服务于过去的两个SCOR 应用程序，并已被证明是一种经济高效的机制，以提供 建议的5个项目中有4个项目的高精度测量 申请。在项目1中，24只转基因小鼠的椎骨和股骨 将在第四年进行测量。这个核心将用于评估骨量 小鼠和大鼠切除的骨骼的变化。活体测量 L4-L6椎体和股骨每年制作3次，共80例。 在整个实验过程中，每年都有小白鼠。小鼠的体外样本 每年将对65只大鼠的股骨和椎骨进行测量 DXA。每年的总样本测量将是580次扫描。在项目4中， 89名患有原发性甲状旁腺功能亢进症的女性将有骨骼 每12个骨骼部位(脊柱、臀部、前臂)进行矿物质测量 几个月的时间来确定骨量的纵向变化 治疗和甲状旁腺切除术后(扫描总数为 这个核心将用于比较hPTH对骨密度的响应 1-34在雌激素缺乏妇女、接受雌激素治疗的妇女和 雌激素组和安慰剂对照组。骨密度对骨密度的影响 HPTH 1-34的终止和稍后的重新挑战也将是 量过了。项目6涉及一系列骨密度测量(重复扫描 在基线和每6个月)在5个骨骼部位(腰椎、髋部、 前臂、全身和侧棘)在治疗过程中，其 退出和后来的重新挑战。所有的扫描总数 项目6中的协议是4900。我们还在核心中加入了一项 确定微型核磁共振是否能提供信息的可行性研究 相当于传统的组织形态计量学，并进一步改进了 在动物模型中骨量预测骨强度的能力。在……里面 除了为这个项目中的个别项目提供核心支持外 格兰特，核心也一直是成功的NIH资助的其他不可或缺的 学习，不增加SCOR补助金的费用。

The bone mineral measurement core is necessary for the measurement of bone mass in both humans and in animals. The core has served the past two SCOR applications and has proven to be a cost effective mechanism of providing high precision measurements for 4 of the 5 projects proposed in this application. In Project 1, the vertebrae and femur of 24 transgenic mice will be measured in year 4. This core will be used to evaluate bone mass changes in mice and the excised bones from rats. In vivo measurements of L4-L6 vertebrae and the femur will be made 3 times per year in 80 live mice per year over the course of the experiment. Ex vivo samples of the femur and vertebrae in 65 rats each year will be measured by utilizing the DXA. Total sample measurements per year will be 580 scans. In Project 4, the cohort of 89 women with primary hyperparathyroidism will have bone mineral measurements at 3 skeletal sites (spine, hip, forearm) every 12 months to determine longitudinal changes in bone mass with medical treatment and following parathyroidectomy (total number of scans is 1068).This core will be used to compare the bone density response to hPTH 1-34 in estrogen deficient women, women on estrogen therapy and women in estrogen and placebo control groups. The bone density response to discontinuation and a later rechallenge of hPTH 1-34 will also be measured. Project 6 involves serial bone density measures (duplicate scans at baseline and every 6 months) at 5 skeletal sites (lumbar spine, hip, forearm, total body, and lateral spine) during the treatment, its' withdrawal and a later rechallenge. The total number of scans for all protocols in Project 6 is 4900. We have also incorporated into the core a feasibility study to determine whether micro-MRI can provide information equivalent to conventional histomorphometry and further improve the ability of bone mass to predict bone strength in the animal model. In addition to providing core support for the individual projects in this grant, the core has also been integral to the success of other NIH funded studies, at no additional cost to the SCOR grant.