CORE--NON-INVASIVE MEASUREMENT OF BONE MINERAL

核心——骨矿物质的无创测量

• 批准号: 6100472
• 负责人: JERI WANZOR NIEVES
• 金额: $ 13.68万
• 依托单位: HELEN HAYES HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-23 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100472
• 关键词: biomedical facility; bone density; bone metabolism; noninvasive diagnosis

The bone mineral measurement core is necessary for the measurement of bone mass in both humans and in animals. The core has served the past two SCOR applications and has proven to be a cost effective mechanism of providing high precision measurements for 4 of the 5 projects proposed in this application. In Project 1, the vertebrae and femur of 24 transgenic mice will be measured in year 4. This core will be used to evaluate bone mass changes in mice and the excised bones from rats. In vivo measurements of L4-L6 vertebrae and the femur will be made 3 times per year in 80 live mice per year over the course of the experiment. Ex vivo samples of the femur and vertebrae in 65 rats each year will be measured by utilizing the DXA. Total sample measurements per year will be 580 scans. In Project 4, the cohort of 89 women with primary hyperparathyroidism will have bone mineral measurements at 3 skeletal sites (spine, hip, forearm) every 12 months to determine longitudinal changes in bone mass with medical treatment and following parathyroidectomy (total number of scans is 1068).This core will be used to compare the bone density response to hPTH 1-34 in estrogen deficient women, women on estrogen therapy and women in estrogen and placebo control groups. The bone density response to discontinuation and a later rechallenge of hPTH 1-34 will also be measured. Project 6 involves serial bone density measures (duplicate scans at baseline and every 6 months) at 5 skeletal sites (lumbar spine, hip, forearm, total body, and lateral spine) during the treatment, its' withdrawal and a later rechallenge. The total number of scans for all protocols in Project 6 is 4900. We have also incorporated into the core a feasibility study to determine whether micro-MRI can provide information equivalent to conventional histomorphometry and further improve the ability of bone mass to predict bone strength in the animal model. In addition to providing core support for the individual projects in this grant, the core has also been integral to the success of other NIH funded studies, at no additional cost to the SCOR grant.

骨矿物质测量核心是测量骨骼所必需的 在人类和动物中的质量。核心服务于过去的两个SCOR 应用程序，并已被证明是一个具有成本效益的机制，提供 高精度测量的5个项目中的4个， 应用程序.在项目1中，24只转基因小鼠的椎骨和股骨 将在第四年进行测量。该核心将用于评估骨量 小鼠和大鼠离体骨的变化。体内测量 L4-L 6椎骨和股骨将在80个活组织中每年制作3次 在整个实验过程中，每年都有老鼠死亡。的离体样品 每年65只大鼠的股骨和椎骨将通过使用 DXA每年的样本测量总数为580次扫描。在项目4中， 89名患有原发性甲状旁腺功能亢进妇女的队列将具有骨 每12天在3个骨骼部位（脊柱、髋关节、前臂）进行矿物质测量 月来确定纵向变化的骨量与医疗 治疗和甲状旁腺切除术后（扫描总数为 1068).该核心将用于比较骨密度对hPTH的反应 1-34在雌激素缺乏妇女、接受雌激素治疗的妇女和 雌激素和安慰剂对照组。骨密度对 停止和稍后再激发hPTH 1-34也将 测定了项目6涉及连续骨密度测量（重复扫描 在基线和每6个月）在5个骨骼部位（腰椎，髋， 前臂、全身和侧脊柱）， 停药和随后的再激发。所有扫描的总次数 项目6的协议是4900。此外，我们亦已将 可行性研究，以确定微型MRI是否可以提供信息 与传统的组织形态计量学相当，并进一步提高 在动物模型中，骨量预测骨强度的能力。在 除了为个别项目提供核心支持外， 该核心项目也是其他NIH资助项目成功的不可或缺的一部分。 研究，在没有额外的成本SCOR赠款。