CORE--NEUROPATHOLOGY AND MOLECULAR DIAGNOSTICS

核心——神经病理学和分子诊断

• 批准号: 6324506
• 负责人: MICHAEL L SHELANSKI
• 金额: $ 27.14万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6324506
• 关键词: Alzheimer's disease; biomedical facility; brain disorder diagnosis; confocal scanning microscopy; cryopreservation; electron microscopy; human genetic material tag; human tissue; meeting /conference /symposium; neuropathology; postmortem; tissue resource /registry; training

The Neuropathology Core and Molecular Diagnostics Core is overseen by a professional staff of Michael L. Shelanski, M.D., Ph.D., and Steven S. Chin, M.D., Ph.D. The core provides "state of the art" neuropathologic examination of patients with various neurodegenerative disorders as well as neurologically normal individuals. These patients are clinically evaluated in detail by the Columbia ADRC and other neurology groups. The core provides detailed reports of findings and diagnoses to patient families and their physicians. Diagnoses are rendered according to presently accepted and recommended neuropathological criteria. In particular, the recently developed NIH-Regan Institute Working Group diagnostic criteria for the neuropathological assessment of Alzheimer's disease is utilized. Standard postmortem handling of brain tissues includes hemisection of the fresh brain, quick freezing of coronally sliced slabs of one half of the brain for banking and eventual distribution to investigators within and outside the ADRC, and fixation of the other half in formalin for histopathological examination and diagnosis. Microscopic examination includes application of H&E, thioflavine S, modified Bieschowsky silver, Gallyas silver, anti-ubiquitin, anti-synuclein, anti- tau, and anti-amyloid stains to selected sections from a standardized set of sample neuroanatomic regions. Collections of formalin-fixed and frozen brain tissue, lymphoblastoid cell lines, DNA from study patients, and their corresponding databases are maintained and distributed to investigators by the core. The core actively educates and trains neuropathology fellows, pathology, neurology, and psychiatry residents, and other health professionals in the importance and practical aspects of post-mortem examination and tissue banking. Clinicopathological correlation conferences are held to review discuss interesting and instructive study cases. The core strongly encourages research collaborations, and providing neuropathological and basic research expertise, access to biologic specimens, and assistance and access to specialized instruments, such as fluorescence, electron and laser capture microscopes as well as high speed DNA sequence analysis.

神经病理学核心和分子诊断核心由Michael L. Shelanski，医学博士，哲学博士、和史蒂文·S. Chin，医学博士，博士该核心提供了“最先进的”神经病理学检查的患者与各种神经退行性疾病以及神经正常的个人。这些患者由哥伦比亚ADRC和其他神经病学小组进行详细的临床评估。核心为患者家属及其医生提供详细的发现和诊断报告。根据目前公认和推荐的神经病理学标准进行诊断。特别是，利用了最近开发的NIH里根研究所工作组用于阿尔茨海默病神经病理学评估的诊断标准。脑组织的标准死后处理包括新鲜脑的半切，将一半脑的冠状切片板快速冷冻以用于储存并最终分发给ADRC内外的研究人员，并将另一半固定在福尔马林中用于组织病理学检查和诊断。显微镜检查包括将H&E、硫代黄素S、改良Bieschowsky银、Gallyas银、抗泛素、抗突触核蛋白、抗tau和抗淀粉样蛋白染色剂应用于来自一组标准化样本神经解剖区域的选定切片。福尔马林固定和冷冻的脑组织、淋巴母细胞系、研究患者的DNA及其相应的数据库的收集由核心维护并分发给研究者。核心积极教育和培训神经病理学研究员，病理学，神经病学和精神病学居民，以及其他卫生专业人员在死后检查和组织库的重要性和实用方面。临床病理相关性会议举行审查讨论有趣和有益的研究案例。核心强烈鼓励研究合作，并提供神经病理学和基础研究专业知识，获得生物标本，并协助和获得专业仪器，如荧光，电子和激光捕获显微镜以及高速DNA序列分析。