CORE--NEUROPATHOLOGY AND MOLECULAR DIAGNOSTICS

神经病理学核心和分子诊断核心由Michael L. Shelanski医学博士和Steven S. Chin医学博士的专业人员监督。该核心为各种神经退行性疾病患者以及神经正常个体提供“最先进的”神经病理学检查。这些患者由哥伦比亚ADRC和其他神经学小组进行详细的临床评估。核心为患者家属和他们的医生提供详细的发现和诊断报告。诊断是根据目前接受和推荐的神经病理标准。特别是，最近制定的NIH-Regan研究所工作组诊断标准用于阿尔茨海默病的神经病理评估。标准的死后脑组织处理包括对新鲜大脑进行半切，快速冷冻冠状切片的一半大脑，用于储存和最终分发给ADRC内外的研究人员，并将另一半大脑在福尔马林中固定，用于组织病理学检查和诊断。显微镜检查包括应用H&E，硫黄素S，修饰Bieschowsky银，galyas银，抗泛素，抗突触核蛋白，抗tau和抗淀粉样蛋白染色从一组标准化的样本神经解剖区域中选择切片。福尔马林固定和冷冻的脑组织、淋巴母细胞系、研究患者的DNA及其相应的数据库由中心维护并分发给研究人员。核心积极教育和培训神经病理学研究员，病理学，神经病学和精神病学住院医师，以及其他卫生专业人员，了解死后检查和组织库的重要性和实践方面。召开临床病理相关会议，回顾讨论有趣和有指导意义的研究病例。该中心强烈鼓励研究合作，并提供神经病理学和基础研究专业知识，获得生物标本，协助和获得专业仪器，如荧光，电子和激光捕获显微镜以及高速DNA序列分析。

The Neuropathology Core and Molecular Diagnostics Core is overseen by a professional staff of Michael L. Shelanski, M.D., Ph.D., and Steven S. Chin, M.D., Ph.D. The core provides "state of the art" neuropathologic examination of patients with various neurodegenerative disorders as well as neurologically normal individuals. These patients are clinically evaluated in detail by the Columbia ADRC and other neurology groups. The core provides detailed reports of findings and diagnoses to patient families and their physicians. Diagnoses are rendered according to presently accepted and recommended neuropathological criteria. In particular, the recently developed NIH-Regan Institute Working Group diagnostic criteria for the neuropathological assessment of Alzheimer's disease is utilized. Standard postmortem handling of brain tissues includes hemisection of the fresh brain, quick freezing of coronally sliced slabs of one half of the brain for banking and eventual distribution to investigators within and outside the ADRC, and fixation of the other half in formalin for histopathological examination and diagnosis. Microscopic examination includes application of H&E, thioflavine S, modified Bieschowsky silver, Gallyas silver, anti-ubiquitin, anti-synuclein, anti- tau, and anti-amyloid stains to selected sections from a standardized set of sample neuroanatomic regions. Collections of formalin-fixed and frozen brain tissue, lymphoblastoid cell lines, DNA from study patients, and their corresponding databases are maintained and distributed to investigators by the core. The core actively educates and trains neuropathology fellows, pathology, neurology, and psychiatry residents, and other health professionals in the importance and practical aspects of post-mortem examination and tissue banking. Clinicopathological correlation conferences are held to review discuss interesting and instructive study cases. The core strongly encourages research collaborations, and providing neuropathological and basic research expertise, access to biologic specimens, and assistance and access to specialized instruments, such as fluorescence, electron and laser capture microscopes as well as high speed DNA sequence analysis.