CORE--BONE MORPHOMETRY AND MOLECULAR CYTOIMAGING

骨形态测量和分子细胞成像核心的目的是 整合关键人员和设备，提供集中资源 加强协作和多学科调查的设施 深入了解骨质疏松症的细胞和分子机制。 核心将 以高质量、可靠且成本低廉的方式执行三种不同的程序 有效方式：骨密度测定、骨组织形态测定 骨组织和骨髓细胞组织染色结合原位 杂交或原位使用逆转录酶-聚合酶链 反应（RT-PCR）。 小鼠骨骼纵向变化的测量 通过双能 X 射线吸收法（DXA 或 DEXA）测定矿物质密度， 我们为此程序开发的技术，用于确定 加速衰老小鼠 (SAM) 和 其杂交后代，骨密度随年龄的变化，性腺切除术和 对小鼠和其他品系的治疗以及离体的最佳时间 未脱钙骨的细胞培养和组织形态学分析 标本。 DEXA 的非侵入性和精确性取代了更多 估计小鼠骨大小的传统方法，例如 灰化或皮质宽度形态测量。 该核心还将获得、修复、 嵌入、切片、染色和量化骨组织形态特征 年轻和年老小鼠的中轴和四肢骨骼，以满足 各种项目。 在本申请的前期工作中， 已经很明显的是，相邻骨的并发性能 RT-PCR 部分用于特定信息和细胞组织染色 标记物，例如抗酒石酸酸性磷酸酶 (TRAP) 破骨细胞、巨噬细胞非特异性酯酶 (NSE) 或碱性 成骨细胞磷酸酶（ALP）与四环素标记骨相结合 需要组织形态计量学来表征骨骼和骨骼的不同子集 骨髓细胞，它们的基因表达模式和活性。 该核心过去已完成 400 多次小鼠体内 DEXA 测量 六个月（从早上 8 点到周末共享使用临床密度计） 至晚上 10 点或工作日下午 5 点后）并已加工 85 块人骨 活检和 60 多个小鼠标本进行组织形态学检查 在过去的一年里。 骨骼实验室是 CAP 认可的设施 安全归档和存储组织和数据的能力。 骨 密度测定和组织形态测定结果将附加到 关系数据库，以便及时返回给主要研究人员 每个项目。

The purpose of the Bone Morphometry and Molecular Cytoimaging Core is to consolidate key personnel and equipment to provide a centralized resource facility to enhance collaborative and multidisciplinary investigations into the cellular and molecular mechanisms of osteoporosis. The Core will perform three different procedures in a high-quality, reliable and cost- effective manner: bone densitometry, bone histomorphometry and histostaining of bone tissue and bone marrow cells combined with in situ hybridization or in situ use of the reverse transcriptase-polymerase chain reaction (RT-PCR). The measurement of longitudinal changes in murine bone mineral density by dual-energy X-ray absorptiometry (DXA or DEXA), a technique we have developed for this Program, is used to determine the differences in bone density in the senescence accelerated mouse (SAM) and its hybrid progeny, the changes in bone density with age, gonadectomy and treatment in thee mice and other strains and the optimal time for ex-vivo cell cultures and histomorphometric analysis of undecalcified bone specimens. The noninvasive and precise nature of DEXA supersedes more traditional methods for the estimation of murine bone magnitude such as ashing or cortical width morphometry. This core will also obtain, fix, embed, section, stain and quantify the bone histomorphometric features of the axial and appendicular skeleton of young and old mice for the needs of the various projects. In preliminary work leading to this application, it has become evident that the concurrent performance on adjacent bone sections of RT-PCR for specific messages and histostaining with cell markers such as tartrate resistant acid phosphatase (TRAP) for osteoclasts, nonspecific esterase (NSE) for macrophages or alkaline phosphatase (ALP) for osteoblasts combined with tetracycline-labeled bone histomorphometry is required to characterize distinct subsets of bone and bone marrow cells, their gene expression patterns and their activity. This core has done over 400 murine DEXA measurements in-vivo over the past six months (sharing use of a clinical densitometer on weekends from 8 A.M. to 10 P.M. or on weekdays after 5 P.M.) and has processed 85 human bone biopsies and over 60 murine specimens for histomorphometric examinations in the past year. The bone laboratory is a CAP accredited facility with the ability to safely archive and store tissue and data. Bone densitometry and histomorphometric results will be appended to a relational database for prompt return to the principal investigators of each project.