CORE--BONE MORPHOMETRY AND MOLECULAR CYTOIMAGING

核心——骨形态学和分子细胞成像

• 批准号: 6295646
• 负责人: ROBERT Stewart WEINSTEIN
• 金额: $ 18.51万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295646
• 关键词: animal tissue; biomedical facility; bone density; bone marrow; histology; human tissue; in situ hybridization; information systems; photon absorptiometry; polymerase chain reaction; tissue /cell culture

The purpose of the Bone Morphometry and Molecular Cytoimaging Core is to consolidate key personnel and equipment to provide a centralized resource facility to enhance collaborative and multidisciplinary investigations into the cellular and molecular mechanisms of osteoporosis. The Core will perform three different procedures in a high-quality, reliable and cost- effective manner: bone densitometry, bone histomorphometry and histostaining of bone tissue and bone marrow cells combined with in situ hybridization or in situ use of the reverse transcriptase-polymerase chain reaction (RT-PCR). The measurement of longitudinal changes in murine bone mineral density by dual-energy X-ray absorptiometry (DXA or DEXA), a technique we have developed for this Program, is used to determine the differences in bone density in the senescence accelerated mouse (SAM) and its hybrid progeny, the changes in bone density with age, gonadectomy and treatment in thee mice and other strains and the optimal time for ex-vivo cell cultures and histomorphometric analysis of undecalcified bone specimens. The noninvasive and precise nature of DEXA supersedes more traditional methods for the estimation of murine bone magnitude such as ashing or cortical width morphometry. This core will also obtain, fix, embed, section, stain and quantify the bone histomorphometric features of the axial and appendicular skeleton of young and old mice for the needs of the various projects. In preliminary work leading to this application, it has become evident that the concurrent performance on adjacent bone sections of RT-PCR for specific messages and histostaining with cell markers such as tartrate resistant acid phosphatase (TRAP) for osteoclasts, nonspecific esterase (NSE) for macrophages or alkaline phosphatase (ALP) for osteoblasts combined with tetracycline-labeled bone histomorphometry is required to characterize distinct subsets of bone and bone marrow cells, their gene expression patterns and their activity. This core has done over 400 murine DEXA measurements in-vivo over the past six months (sharing use of a clinical densitometer on weekends from 8 A.M. to 10 P.M. or on weekdays after 5 P.M.) and has processed 85 human bone biopsies and over 60 murine specimens for histomorphometric examinations in the past year. The bone laboratory is a CAP accredited facility with the ability to safely archive and store tissue and data. Bone densitometry and histomorphometric results will be appended to a relational database for prompt return to the principal investigators of each project.

骨形态测量和分子细胞成像核心的目的是 整合关键人员和设备，提供集中资源 加强合作和多学科调查的设施 骨质疏松症的细胞和分子机制。 芯会 执行三个不同的程序在一个高品质，可靠和成本- 有效方法：骨密度测定、骨组织形态测定和 骨组织和骨髓细胞组织染色结合原位 杂交或原位使用逆转录酶-聚合酶链 反应（RT-PCR）。 小鼠骨纵向变化的测量 双能X线吸收法（DXA或DEXA）测定矿物质密度，a 我们为该计划开发的技术，用于确定 加速衰老小鼠（SAM）骨密度的差异， 其杂交后代，骨密度随年龄的变化，性腺切除， 在三种小鼠和其他品系中的治疗和离体的最佳时间 不脱钙骨的细胞培养和组织形态计量学分析 标本 DEXA的非侵入性和精确性取代了更多 用于估计鼠骨大小的传统方法， 灰化或皮质宽度形态测定法。 这个核心还将获得，修复， 包埋、切片、染色和定量骨组织形态计量学特征， 青年和老年小鼠的中轴和四肢骨骼， 各种项目。 在这项申请的初步工作中， 已经变得明显的是， RT-PCR切片以获取特定信息并进行细胞组织染色 抗酒石酸酸性磷酸酶（TRAP） 破骨细胞，巨噬细胞或碱性非特异性酯酶（NSE） 成骨细胞碱性磷酸酶（ALP）与四环素标记骨结合 需要组织形态测量来表征骨的不同子集， 骨髓细胞，它们的基因表达模式和它们的活性。 过去，该核心已经在体内进行了400多次小鼠DEXA测量 六个月（周末从上午8点开始共用一台临床密度计， 至晚上10点或工作日下午5点后）已经处理了85块人骨 活组织检查和60多个用于组织形态学检查的小鼠标本 过去一年 骨实验室是CAP认证的机构， 安全存档和存储组织和数据的能力。 骨 密度测定和组织形态测定结果将随附于 关系数据库，以便迅速返回给主要研究者， 每个项目。